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Title: Fluorescence microscopy: A tool to study autophagy
Authors: Rai, Shashank
Manjithaya, Ravi
Keywords: Nanoscience & Nanotechnology
Materials Science
Applied Physics
Monitoring Autophagy
Tagged Lc3
Issue Date: 2015
Publisher: American Institute of Physics
Citation: AIP Advances
Rai, S.; Manjithaya, R., Fluorescence microscopy: A tool to study autophagy. AIP Advances 2015, 5 (8), 8.
Abstract: Autophagy is a cellular recycling process through which a cell degrades old and damaged cellular components such as organelles and proteins and the degradation products are reused to provide energy and building blocks. Dysfunctional autophagy is reported in several pathological situations. Hence, autophagy plays an important role in both cellular homeostasis and diseased conditions. Autophagy can be studied through various techniques including fluorescence based microscopy. With the advancements of newer technologies in fluorescence microscopy, several novel processes of autophagy have been discovered which makes it an essential tool for autophagy research. Moreover, ability to tag fluorescent proteins with sub cellular targets has enabled us to evaluate autophagy processes in real time under fluorescent microscope. In this article, we demonstrate different aspects of autophagy in two different model organisms i.e. yeast and mammalian cells, with the help of fluorescence microscopy. (C) 2015 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
Description: Restricted access
ISSN: 2158-3226
Appears in Collections:Research Papers (Ravi Manjithaya)

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