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Title: Sequence-specific recognition of DNA minor groove by an NIR-fluorescence switch-on probe and its potential applications
Authors: Narayanaswamy, Nagarjun
Das, Shubhajit
Samanta, Pralok K.
Banu, Khadija
Sharma, Guru Prasad
Mondal, Neelima
Dhar, Suman K.
Pati, Swapan Kumar
Govindaraju, T.
Keywords: Biochemistry & Molecular Biology
Double-Stranded DNA
Malaria Parasites
Living Cells
Cyanine Dyes
Issue Date: 2015
Publisher: Oxford Univ Press
Citation: Nucleic Acids Research
Narayanaswamy, N.; Das, S.; Samanta, P. K.; Banu, K.; Sharma, G. P.; Mondal, N.; Dhar, S. K.; Pati, S. K.; Govindaraju, T., Sequence-specific recognition of DNA minor groove by an NIR-fluorescence switch-on probe and its potential applications. Nucleic Acids Res. 2015, 43 (18), 8651-8663.
Abstract: In molecular biology, understanding the functional and structural aspects of DNA requires sequence-specific DNA binding probes. Especially, sequence-specific fluorescence probes offer the advantage of real-time monitoring of the conformational and structural reorganization of DNA in living cells. Herein, we designed a new class of D2A (one-donor-two-acceptor) near-infrared (NIR) fluorescence switch-on probe named quinone cyanine-dithiazole (QCy-DT) based on the distinctive internal charge transfer (ICT) process for minor groove recognition of AT-rich DNA. Interestingly, QCy-DT exhibited strong NIR-fluorescence enhancement in the presence of AT-rich DNA compared to GC-rich and single-stranded DNAs. We show sequence-specific minor groove recognition of QCy-DT for DNA containing 5'-AATT-3' sequence over other variable (A/T) 4 sequences and local nucleobase variation study around the 5'-X(AATT) Y-3' recognition sequence revealed that X = A and Y = T are the most preferable nucleobases. The live cell imaging studies confirmed mammalian cell permeability, low-toxicity and selective staining capacity of nuclear DNA without requiring RNase treatment. Further, Plasmodium falciparum with an AT-rich genome showed specific uptake with a reasonably low IC50 value (< 4 mu M). The ease of synthesis, large Stokes shift, sequence-specific DNA minor groove recognition with switch-on NIR-fluorescence, photostability and parasite staining with low IC50 make QCy-DT a potential and commercially viable DNA probe.
Description: Restricted access
ISSN: 0305-1048
Appears in Collections:Research Articles (Swapan Kumar Pati)

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