Please use this identifier to cite or link to this item:
https://libjncir.jncasr.ac.in/xmlui/handle/10572/2048
Title: | Sequence-specific recognition of DNA minor groove by an NIR-fluorescence switch-on probe and its potential applications |
Authors: | Narayanaswamy, Nagarjun Das, Shubhajit Samanta, Pralok K. Banu, Khadija Sharma, Guru Prasad Mondal, Neelima Dhar, Suman K. Pati, Swapan Kumar Govindaraju, T. |
Keywords: | Biochemistry & Molecular Biology Double-Stranded DNA Molecular-Dynamics Optical-Properties Malaria Parasites Crystal-Structure Water-Molecules Living Cells Cyanine Dyes Base-Pairs Binding |
Issue Date: | 2015 |
Publisher: | Oxford Univ Press |
Citation: | Nucleic Acids Research 43 18 Narayanaswamy, N.; Das, S.; Samanta, P. K.; Banu, K.; Sharma, G. P.; Mondal, N.; Dhar, S. K.; Pati, S. K.; Govindaraju, T., Sequence-specific recognition of DNA minor groove by an NIR-fluorescence switch-on probe and its potential applications. Nucleic Acids Res. 2015, 43 (18), 8651-8663. |
Abstract: | In molecular biology, understanding the functional and structural aspects of DNA requires sequence-specific DNA binding probes. Especially, sequence-specific fluorescence probes offer the advantage of real-time monitoring of the conformational and structural reorganization of DNA in living cells. Herein, we designed a new class of D2A (one-donor-two-acceptor) near-infrared (NIR) fluorescence switch-on probe named quinone cyanine-dithiazole (QCy-DT) based on the distinctive internal charge transfer (ICT) process for minor groove recognition of AT-rich DNA. Interestingly, QCy-DT exhibited strong NIR-fluorescence enhancement in the presence of AT-rich DNA compared to GC-rich and single-stranded DNAs. We show sequence-specific minor groove recognition of QCy-DT for DNA containing 5'-AATT-3' sequence over other variable (A/T) 4 sequences and local nucleobase variation study around the 5'-X(AATT) Y-3' recognition sequence revealed that X = A and Y = T are the most preferable nucleobases. The live cell imaging studies confirmed mammalian cell permeability, low-toxicity and selective staining capacity of nuclear DNA without requiring RNase treatment. Further, Plasmodium falciparum with an AT-rich genome showed specific uptake with a reasonably low IC50 value (< 4 mu M). The ease of synthesis, large Stokes shift, sequence-specific DNA minor groove recognition with switch-on NIR-fluorescence, photostability and parasite staining with low IC50 make QCy-DT a potential and commercially viable DNA probe. |
Description: | Restricted access |
URI: | https://libjncir.jncasr.ac.in/xmlui/10572/2048 |
ISSN: | 0305-1048 |
Appears in Collections: | Research Articles (Swapan Kumar Pati) |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.