Please use this identifier to cite or link to this item: https://libjncir.jncasr.ac.in/xmlui/handle/10572/2189
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dc.contributor.authorShetty, Deeti K.
dc.contributor.authorShetty, Ronak
dc.contributor.authorInamdar, Maneesha S.
dc.date.accessioned2017-01-24T06:32:23Z-
dc.date.available2017-01-24T06:32:23Z-
dc.date.issued2016
dc.identifier.citationShetty, D. K.; Shetty, R.; Inamdar, M. S., Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On. Stem Cell Research 2016, 16 (2), 246-248 http://dx.doi.org/10.1016/j.scr.2015.12.045en_US
dc.identifier.citationStem Cell Researchen_US
dc.identifier.citation16en_US
dc.identifier.citation2en_US
dc.identifier.issn1873-5061
dc.identifier.urihttps://libjncir.jncasr.ac.in/xmlui/10572/2189-
dc.descriptionRestricted Accessen_US
dc.description.abstractHuman embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On cells can be used for inducible expression of any coding sequence cloned into pTRE-Tight vector. For example, in human embryonic stem cells, Tet-On system has been used to generate SOX2 overexpression cell line (Adachi et al., 2010). (C) 2016 The Authors. Published by Elsevier B.V.en_US
dc.description.uri1876-7753en_US
dc.description.urihttp://dx.doi.org/10.1016/j.scr.2015.12.045en_US
dc.language.isoEnglishen_US
dc.publisherElsevier Science Bven_US
dc.rights@Elsevier Science Bv, 2016en_US
dc.subjectCell Biologyen_US
dc.subjectBiotechnology & Applied Microbiologyen_US
dc.titleGeneration of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-Onen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (Maneesha S. Inamdar)

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