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  4. Maneesha S. Inamdar
  5. Research Papers (Maneesha S. Inamdar)
Please use this identifier to cite or link to this item: https://libjncir.jncasr.ac.in/xmlui/handle/10572/2190
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dc.contributor.authorShetty, Ronak
dc.contributor.authorInamdar, Maneesha S.
dc.date.accessioned2017-01-24T06:32:23Z-
dc.date.available2017-01-24T06:32:23Z-
dc.date.issued2016
dc.identifier.citationShetty, R.; Inamdar, M. S., Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR. Stem Cell Research 2016, 16 (2), 271-273 http://dx.doi.org/10.1016/j.scr.2015.12.043en_US
dc.identifier.citationStem Cell Researchen_US
dc.identifier.citation16en_US
dc.identifier.citation2en_US
dc.identifier.issn1873-5061
dc.identifier.urihttps://libjncir.jncasr.ac.in/xmlui/10572/2190-
dc.descriptionRestricted Accessen_US
dc.description.abstractHuman embryonic stem cell line BJNhem20-TetR was generated using non-viral method. The construct pCAG-TetRnls was transfected using microporation procedure. BJNhem20-TetR can subsequently be transfected with any vector harbouring a TetO (Tet operator) sequence to generate doxycycline based inducible line. For example, in human embryonic stem cells, the pSuperior based TetO system has been transfected into a TetR containing line to generate OCT4 knockdown cell line (Zafarana et al., 2009). Thus BJNhem20-TetR can be used as a tool to perturb gene expression in human embryonic stem cells. (C) 2016 The Authors. Published by Elsevier B.V.en_US
dc.description.uri1876-7753en_US
dc.description.urihttp://dx.doi.org/10.1016/j.scr.2015.12.043en_US
dc.language.isoEnglishen_US
dc.publisherElsevier Science Bven_US
dc.rights@Elsevier Science Bv, 2016en_US
dc.subjectCell Biologyen_US
dc.subjectBiotechnology & Applied Microbiologyen_US
dc.titleGeneration of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetRen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (Maneesha S. Inamdar)

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