Please use this identifier to cite or link to this item: https://libjncir.jncasr.ac.in/xmlui/handle/10572/2301
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dc.contributor.authorHoque, Jiaul
dc.contributor.authorAkkapeddi, Padma
dc.contributor.authorGhosh, Chandradhish
dc.contributor.authorUppu, Divakara S. S. M.
dc.contributor.authorHaldar, Jayanta
dc.date.accessioned2017-01-24T09:11:14Z-
dc.date.available2017-01-24T09:11:14Z-
dc.date.issued2016
dc.identifier.citationHoque, J.; Akkapeddi, P.; Ghosh, C.; Uppu, Dssm; Haldar, J., A Biodegradable Polycationic Paint that Kills Bacteria in Vitro and in Vivo. Acs Applied Materials & Interfaces 2016, 8 (43), 29298-29309 http://dx.doi.org/10.1021/acsami.6b09804en_US
dc.identifier.citationACS Applied Materials & Interfacesen_US
dc.identifier.citation8en_US
dc.identifier.citation43en_US
dc.identifier.issn1944-8244
dc.identifier.urihttps://libjncir.jncasr.ac.in/xmlui/10572/2301-
dc.descriptionOpen Access (Accepted Manuscript)en_US
dc.description.abstractBacterial colonization and subsequent formation of biofilms onto surfaces of medical devices and implants is a major source of nosocomial infections. Most antibacterial coatings to combat infections are either metal-based or nondegradable polymer-based and hence limited by their nondegradability and unpredictable toxicity. Moreover, to combat infections effectively, the coatings are required to display simultaneous antibacterial and antibiofilm activity. Herein we report biocompatible and biodegradable coatings based on organo-soluble quaternary chitin polymers which were immobilized noncovalently onto surfaces as bactericidal paint. The polycationic paint was found to be active against both drug-sensitive and-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), and beta-lactam-resistant Klebsiella pneumoniae. The cationic polymers were shown to interact with the negatively charged bacterial cell membrane and disrupt the membrane integrity, thereby causing leakage of intracellular constituents and cell death upon contact. Importantly, surfaces coated with the polymers inhibited formation of biofilms against both Gram-positive S. aureus and Gram-negative E. coli, two of the most clinically important bacteria that form biofilms. Surfaces coated with the polymers displayed negligible toxicity against human erythrocytes and embryo kidney cells. Notably, the polymers were shown to be susceptible toward lysozyme. Furthermore, subcutaneous implantation of polymer discs in rats led to 15-20% degradation in 4 weeks thereby displaying their biodegradability. In a murine model of subcutaneous infection, polymer-coated medical-grade catheter reduced MRSA burden by 3.7 log compared to that of noncoated catheter. Furthermore, no biofilm development was observed on the coated catheters under in vivo conditions. The polycationic materials thus developed herein represent a novel class of safe and effective coating agents for the prevention of device-associated infections.en_US
dc.description.urihttp://dx.doi.org/10.1021/acsami.6b09804en_US
dc.language.isoEnglishen_US
dc.publisherAmerican Chemical Societyen_US
dc.rights@American Chemical Society, 2016en_US
dc.subjectMaterials Scienceen_US
dc.subjectbiodegradable bactericidal painten_US
dc.subjectanti-infective biomaterialsen_US
dc.subjectdrug-resistant bacteriaen_US
dc.subjectbiofilm inhibitionen_US
dc.subjectorgano-soluble quaternary chitin derivativesen_US
dc.subjectBroad-Spectrum Antibacterialen_US
dc.subjectEradicate Biofilmsen_US
dc.subjectSmall Moleculesen_US
dc.subjectOne-Stepen_US
dc.subjectCoatingsen_US
dc.subjectResistanceen_US
dc.subjectSurfaceen_US
dc.subjectInfectionen_US
dc.subjectReleaseen_US
dc.subjectFilmsen_US
dc.titleA Biodegradable Polycationic Paint that Kills Bacteria in Vitro and in Vivoen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (Jayanta Haldar)

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