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Title: Evaluation of chitosan nanoformulations as potent anti-HIV therapeutic systems
Authors: Ramana, Lakshmi Narashimhan
Sharma, Shilpee
Sethuraman, Swaminathan
Ranga, Udaykumar
Krishnan, Uma Maheswari
Keywords: Biochemistry & Molecular Biology
Solid Lipid Nanoparticles
Issue Date: 2014
Publisher: Elsevier Science Bv
Citation: Ramana, LN; Sharma, S; Sethuraman, S; Ranga, U; Krishnan, UM, Evaluation of chitosan nanoformulations as potent anti-HIV therapeutic systems. Biochimica Et Biophysica Acta-General Subjects 2014, 1840 (1) 476-484,
Biochimica Et Biophysica Acta-General Subjects
Abstract: Background: Antiretroviral Therapy (ART) is currently the major therapeutic intervention in the treatment of AIDS. ART, however, is severely limited due to poor availability, high cytotoxicity, and enhanced metabolism and clearance of the drug molecules by the renal system. The use of nanocarriers encapsulating the antiretroviral drugs may provide a solution to the aforementioned problems. Importantly, the application of mildly immunogenic polymeric carrier confers the advantage of making the nanoparticles more visible to the immune system leading to their efficient uptake by the phagocytes. Methods: The saquinavir-loaded chitosan nanopartides were characterized by transmission electron microscopy and differential scanning calorimetry and analyzed for the encapsulation efficiency, swelling characteristics, particle size properties, and the zeta potential. Furthermore, cellular uptake of the chitosan nanocarriers was evaluated using confocal microscopy and Flow cytometry. The antiviral efficacy was quantified using viral infection of the target cells. Results: Using novel chitosan carriers loaded with saquinavir, a protease inhibitor, we demonstrate a drug encapsulation efficiency of 75% and cell targeting efficiency greater than 92%. As compared to the soluble drug control, the saquinavir-loaded chitosan carriers caused superior control of the viral proliferation as measured by using two different viral strains, NL4-3 and Indie-C1, and two different target T-cells, Jurkat and CEM-CCR5. Conclusion: Chitosan nanoparticles loaded with saquinavir were characterized and they demonstrated superior drug loading potential with greater cell targeting efficiency leading to efficient control of the viral proliferation in target T-cells. General significance: Our data ascertain the potential of chitosan nanocarriers as novel vehicles for HIV-1 therapeutics. (C) 2013 Elsevier B.V. All rights reserved.
Description: Restricted Access
ISSN: 0304-4165
Appears in Collections:Research Papers (Ravi Manjithaya)

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