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dc.contributor.authorRavindra, Kodihalli C-
dc.contributor.authorSelvi, B Ruthrotha-
dc.contributor.authorArif, Mohammed-
dc.contributor.authorReddy, B A Ashok-
dc.contributor.authorThanuja, Gali R-
dc.contributor.authorAgrawal, Shipra-
dc.contributor.authorPradhan, Suman Kalyan-
dc.contributor.authorNagashayana, Natesh-
dc.contributor.authorDasgupta, Dipak-
dc.contributor.authorKundu, Tapas K-
dc.date.accessioned2012-02-14T06:46:20Z-
dc.date.available2012-02-14T06:46:20Z-
dc.date.issued2009-09-04-
dc.identifier0021-9258en_US
dc.identifier.citationJournal Of Biological Chemistry 284(36), 24453-24464 (2009)en_US
dc.identifier.urihttps://libjncir.jncasr.ac.in/xmlui/10572/400-
dc.descriptionRestricted Accessen_US
dc.description.abstractLysine acetyltransferases (KATs), p300 (KAT3B), and its close homologue CREB-binding protein (KAT3A) are probably the most widely studied KATs with well documented roles in various cellular processes. Hence, the dysfunction of p300 may result in the dysregulation of gene expression leading to the manifestation of many disorders. The acetyltransferase activity of p300/CREB-binding protein is therefore considered as a target for new generation therapeutics. We describe here a natural compound, plumbagin (RTK1), isolated from Plumbago rosea root extract, that inhibits histone acetyltransferase activity potently in vivo. Interestingly, RTK1 specifically inhibits the p300-mediated acetylation of p53 but not the acetylation by another acetyltransferase, p300/CREB-binding protein-associated factor, PCAF, in vivo. RTK1 inhibits p300 histone acetyltransferase activity in a noncompetitive manner. Docking studies and site-directed mutagenesis of the p300 histone acetyltransferase domain suggest that a single hydroxyl group of RTK1 makes a hydrogen bond with the lysine 1358 residue of this domain. In agreement with this, we found that indeed the hydroxyl group-substituted plumbagin derivatives lost the acetyltransferase inhibitory activity. This study describes for the first time the chemical entity (hydroxyl group) required for the inhibition of acetyltransferase activity.en_US
dc.description.sponsorshipDepartment of Biotechnology, Government of India. Jawaharlal Nehru Centre for Advanced Scientific Research.en_US
dc.description.urihttp://dx.doi.org/10.1074/jbc.M109.023861en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology Incen_US
dc.rights© 2009 The American Society for Biochemistry and Molecular Biology Incen_US
dc.subjectP300 Histone Acetyltransferaseen_US
dc.subjectEnhanced Raman-Spectroscopyen_US
dc.subjectGlobal Gene- Expressionen_US
dc.subjectTranscriptional Coactivatoren_US
dc.subjectCancer Cellsen_US
dc.subjectChromatin Transcriptionen_US
dc.subjectProtein Acetylationen_US
dc.subjectSmall Moleculesen_US
dc.subjectHuman-Diseaseen_US
dc.subjectDna-Damageen_US
dc.titleInhibition of Lysine Acetyltransferase KAT3B/p300 Activity by a Naturally Occurring Hydroxynaphthoquinone, Plumbaginen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (Tapas K. Kundu)

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