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Browsing Research Papers (Maneesha S. Inamdar) by Author

Browsing Research Papers (Maneesha S. Inamdar) by Author "Inamdar, Maneesha S."

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ARF1-GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis

Khadilkar, Rohan J.; Rodrigues, Diana; Mote, Ridim Dadasaheb; Sinha, Arghyashree Roychowdhury; Kulkarni, Vani; Magadi, Srivathsa Subramanya; Inamdar, Maneesha S. (National Academy of Sciences, 2014)

Drosophila melanogaster larval hematopoiesis is a well-established model to study mechanisms that regulate hematopoietic niche maintenance and control of blood cell precursor (prohemocyte) differentiation. Molecules that ...
Controlled Inhibition of the Mesenchymal Stromal Cell Pro-inflammatory Secretome via Microparticle Engineering

Ranganath, Sudhir H.; Tong, Zhixiang; Levy, Oren; Martyn, Keir; Karp, Jeffrey M.; Inamdar, Maneesha S. (Cell Press, 2016)

Mesenchymal stromal cells (MSCs) are promising therapeutic candidates given their potent immunomodulatory and anti-inflammatory secretome. However, controlling the MSC secretome post-transplantation is considered a major ...
Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR

Shetty, Ronak; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Human embryonic stem cell line BJNhem20-TetR was generated using non-viral method. The construct pCAG-TetRnls was transfected using microporation procedure. BJNhem20-TetR can subsequently be transfected with any vector ...
Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-20

Shetty, Deeti K.; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Ovarian carcinoma immuno-reactive antigen domain containing 1(OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-20. The line was generated ...
Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39

Shetty, Deeti K.; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Ovarian carcinoma immuno-reactive antigen domain containing 1 (OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-39. The line was generated ...
Generation of a transgenic human embryonic stem cell line ectopically expressing the endosomal protein Asrij that regulates pluripotency in mouse embryonic stem cells: BJNhem20-Asrij

Shetty, Deeti K.; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Asrij is an endocytic protein expressed in mouse embryonic stem cells (mESCs) and is essential for maintenance of stemness of mESCs (Mukhopadhyay et al., 2003; Sinha et al., 2013). Its human ortholog named Ovarian Carcinoma ...
Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato

Shetty, Deeti K.; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity ...
Generation of human embryonic stem cell line expressing green fluorescent protein

Shetty, Ronak; Shetty, Deeti; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Human embryonic stem cell line BJNhem20 pCAG-EGFP was generated using non-viral method (Fig. 1). The construct pCAG-EGFP was transfected using microporation procedure (C) 2015 The Authors. Published by Elsevier B.V.
Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On

Shetty, Deeti K.; Shetty, Ronak; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Human embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On ...
Generation of transgenic human embryonic stem cell line BJNhem20-OCIAD1-OV

Shetty, Deeti K.; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Ovarian Carcinoma Immuno-reactive Antigen domain containing protein 1 (OCIAD1) was overexpressed in BJNhem20 human embryonic stem cell line (hESC) using plasmid transfection, followed by stable cell line generation. The ...

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