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An impediment to random walk: trehalose microenvironment drives preferential endocytic uptake of plasmonic nanoparticles

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dc.contributor.author Siddhanta, Soumik
dc.contributor.author Zheng, Chao
dc.contributor.author Narayana, Chandrabhas
dc.contributor.author Barman, Ishan
dc.date.accessioned 2017-01-24T06:17:35Z
dc.date.available 2017-01-24T06:17:35Z
dc.date.issued 2016
dc.identifier.citation Siddhanta, S.; Zheng, C.; Narayana, C.; Barman, I., An impediment to random walk: trehalose microenvironment drives preferential endocytic uptake of plasmonic nanoparticles. Chemical Science 2016, 7 (6), 3730-3736 http://dx.doi.org/10.1039/c6sc00510a en_US
dc.identifier.citation Chemical Science en_US
dc.identifier.citation 7 en_US
dc.identifier.citation 6 en_US
dc.identifier.issn 2041-6520
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/2072
dc.description Open Access en_US
dc.description.abstract Developing effective theranostic nanoplex platforms for personalized disease treatment necessitates an understanding of and the ability to control live cell-nanoparticle interactions. However, aggregation of nanoparticles on the cell surface and their subsequent internalization is sparsely understood and adversely impact cellular recognition and viability. Here we report a facile method of precisely modulating the aggregation and uptake for silver nanoparticles without altering their surface geometry or functionalization. Exploiting the stabilization properties of trehalose, our approach enables uptake of nanoparticles while reducing aggregation on cell surface and maintaining cell viability. Electron microscopy reveals the larger utilization of endosomal structures in the trehalose-rich environment compared to the nanoparticles' "free" cytosolic diffusion patterns in the control group. Additionally, in the presence of trehalose, plasmon-enhanced Raman spectroscopy confirms the preservation of the protein structure in the vicinity of the nanoparticles reinforcing the promise of the proposed route for label-free, multiplexed intracellular monitoring. en_US
dc.description.uri 2041-6539 en_US
dc.description.uri http://dx.doi.org/10.1039/c6sc00510a en_US
dc.language English en
dc.language.iso English en_US
dc.publisher Royal Society of Chemistry en_US
dc.rights @Royal Society of Chemistry, 2016 en_US
dc.subject Chemistry en_US
dc.subject Surface-Enhanced Raman en_US
dc.subject Gold Nanoparticles en_US
dc.subject Silver Nanoparticles en_US
dc.subject Protein Corona en_US
dc.subject Cells en_US
dc.subject Spectroscopy en_US
dc.subject Cytotoxicity en_US
dc.subject Aggregation en_US
dc.subject Adsorption en_US
dc.subject Toxicity en_US
dc.title An impediment to random walk: trehalose microenvironment drives preferential endocytic uptake of plasmonic nanoparticles en_US
dc.type Article en_US


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