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Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato

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dc.contributor.author Shetty, Deeti K.
dc.contributor.author Inamdar, Maneesha S.
dc.date.accessioned 2017-01-24T06:32:22Z
dc.date.available 2017-01-24T06:32:22Z
dc.date.issued 2016
dc.identifier.citation Shetty, D. K.; Inamdar, M. S., Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato. Stem Cell Research 2016, 16 (2), 290-292 http://dx.doi.org/10.1016/j.scr.2015.12.046 en_US
dc.identifier.citation Stem Cell Research en_US
dc.identifier.citation 16 en_US
dc.identifier.citation 2 en_US
dc.identifier.issn 1873-5061
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/2185
dc.description Restricted Access en_US
dc.description.abstract Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity within individual cells in hESC colonies by enabling live tracking of their growth, migration and differentiation properties. This cell line also serves as a resource for additional transgene introduction/knock-out/knock-in generation in a fluorescent background and allows ease of analysis in studies involving cell mixing. (C) 2016 The Authors. Published by Elsevier B.V. en_US
dc.description.uri 1876-7753 en_US
dc.description.uri http://dx.doi.org/10.1016/j.scr.2015.12.046 en_US
dc.language.iso English en_US
dc.publisher Elsevier Science Bv en_US
dc.rights @Elsevier Science Bv, 2016 en_US
dc.subject Cell Biology en_US
dc.subject Biotechnology & Applied Microbiology en_US
dc.title Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato en_US
dc.type Article en_US


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