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Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On
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| dc.contributor.author | Shetty, Deeti K.
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| dc.contributor.author | Shetty, Ronak
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| dc.contributor.author | Inamdar, Maneesha S.
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| dc.date.accessioned | 2017-01-24T06:32:23Z | |
| dc.date.available | 2017-01-24T06:32:23Z | |
| dc.date.issued | 2016 | |
| dc.identifier.citation | Shetty, D. K.; Shetty, R.; Inamdar, M. S., Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On. Stem Cell Research 2016, 16 (2), 246-248 http://dx.doi.org/10.1016/j.scr.2015.12.045 | en_US |
| dc.identifier.citation | Stem Cell Research | en_US |
| dc.identifier.citation | 16 | en_US |
| dc.identifier.citation | 2 | en_US |
| dc.identifier.issn | 1873-5061 | |
| dc.identifier.uri | https://libjncir.jncasr.ac.in/xmlui/10572/2189 | |
| dc.description | Restricted Access | en_US |
| dc.description.abstract | Human embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On cells can be used for inducible expression of any coding sequence cloned into pTRE-Tight vector. For example, in human embryonic stem cells, Tet-On system has been used to generate SOX2 overexpression cell line (Adachi et al., 2010). (C) 2016 The Authors. Published by Elsevier B.V. | en_US |
| dc.description.uri | 1876-7753 | en_US |
| dc.description.uri | http://dx.doi.org/10.1016/j.scr.2015.12.045 | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Elsevier Science Bv | en_US |
| dc.rights | @Elsevier Science Bv, 2016 | en_US |
| dc.subject | Cell Biology | en_US |
| dc.subject | Biotechnology & Applied Microbiology | en_US |
| dc.title | Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On | en_US |
| dc.type | Article | en_US |
