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Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR
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| dc.contributor.author | Shetty, Ronak
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| dc.contributor.author | Inamdar, Maneesha S.
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| dc.date.accessioned | 2017-01-24T06:32:23Z | |
| dc.date.available | 2017-01-24T06:32:23Z | |
| dc.date.issued | 2016 | |
| dc.identifier.citation | Shetty, R.; Inamdar, M. S., Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR. Stem Cell Research 2016, 16 (2), 271-273 http://dx.doi.org/10.1016/j.scr.2015.12.043 | en_US |
| dc.identifier.citation | Stem Cell Research | en_US |
| dc.identifier.citation | 16 | en_US |
| dc.identifier.citation | 2 | en_US |
| dc.identifier.issn | 1873-5061 | |
| dc.identifier.uri | https://libjncir.jncasr.ac.in/xmlui/10572/2190 | |
| dc.description | Restricted Access | en_US |
| dc.description.abstract | Human embryonic stem cell line BJNhem20-TetR was generated using non-viral method. The construct pCAG-TetRnls was transfected using microporation procedure. BJNhem20-TetR can subsequently be transfected with any vector harbouring a TetO (Tet operator) sequence to generate doxycycline based inducible line. For example, in human embryonic stem cells, the pSuperior based TetO system has been transfected into a TetR containing line to generate OCT4 knockdown cell line (Zafarana et al., 2009). Thus BJNhem20-TetR can be used as a tool to perturb gene expression in human embryonic stem cells. (C) 2016 The Authors. Published by Elsevier B.V. | en_US |
| dc.description.uri | 1876-7753 | en_US |
| dc.description.uri | http://dx.doi.org/10.1016/j.scr.2015.12.043 | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Elsevier Science Bv | en_US |
| dc.rights | @Elsevier Science Bv, 2016 | en_US |
| dc.subject | Cell Biology | en_US |
| dc.subject | Biotechnology & Applied Microbiology | en_US |
| dc.title | Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR | en_US |
| dc.type | Article | en_US |
