Melatonin Enhances the Sensitivity of Circadian Pacemakers to Light in the Nocturnal Field Mouse Mus booduga

Abstract

The effect of exogenous melatonin (1 mg/kg) on light pulse (LP) induced phase shifts of the circadian locomotor activity rhythm was studied in the nocturnal field mouse Mus booduga. Three phase response curves (PRCs: LP, control, and experimental) were constructed to study the effect of co-administration of light and melatonin at various circadian times (CTs). The LP PRC was constructed by exposing animals free-running in constant darkness (DD) to LPs of 100-lux intensity and 15-min duration, at various CTs. The control and experimental PRCs were constructed by using a single injection of either 50% DMSO or melatonin (1 mg/kg dissolved in 50% DMSO), respectively, administered 5 min before LPs, to animals free-running in DD. A single dose of melatonin significantly modified the waveform of the LP PRC. The experimental PRC had significantly larger areas under advance and delay regions of the PRC compared to the control PRC. This was also confirmed when the phase shifts obtained at various CTs were compared between the three PRCs. The phase delays at three phases (CT12, CT14, and CT16) of the experimental PRCs were significantly greater than those of the control and the LP PRCs. Based on these results we conclude that phase shifting effects of melatonin and light add up to produce larger responses. (C) 2003 Wiley-Liss, Inc.