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Effects of temporally controlled huntingtin expression and autophagy upregulation in drosophila melanogaster

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dc.contributor.advisor Vasu, Sheeba
dc.contributor.author Ganguly, Payel
dc.date.accessioned 2019-07-18T11:04:36Z
dc.date.available 2019-07-18T11:04:36Z
dc.date.issued 2015-04-06
dc.identifier.citation Ganguly, Payel. 2015, Effects of temporally controlled huntingtin expression and autophagy upregulation in drosophila melanogaster, MS thesis, Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru en_US
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/handle/10572/2628
dc.description.abstract Previous studies in the laboratory have shown that expression of pathogenic human Huntingtin (HTT-Q128) in circadian pacemaker neurons (ventro lateral neurons, LNv) of Drosophila, leads to immediate behavioural arrhythmicity in constant darkness and loss of neuropeptide Pigment Dispersing Factor (PDF) specifically from a subset of LNv - the small LNv (sLNv), while the large LNv (lLNv) are intact. In the present study, we have attempted to examine effects of temporally restricting HTT expression in LNv on neuronal function and behaviour using an inducible Gene Switch system that enables HTT induction only in the presence of a progesterone analogue RU. We used this system to ask whether larval versus adult specific HTT induction alters the selective vulnerability of sLNv and circadian behavioural dysfunction (arrhythmic locomotor activity) under constant darkness. It was seen that either adult-restricted or larval-restricted HTT induction were each sufficient to render flies arrhythmic, albeit to different magnitudes. Moreover, the onset of arrhythmicity was delayed compared to flies expressing HTT throughout. Both these treatments were also sufficient to cause loss of PDF in sLNv; but at a slower rate compared to flies expressing HTT throughout. Therefore, the selective susceptibility of sLNv does not seem to depend on the life-stage at which HTT is expressed, but the rate of loss seems to be affected by the duration of HTT expression. en_US
dc.language.iso English en_US
dc.publisher Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) en_US
dc.rights © 2015 JNCASR
dc.subject Drosophila melanogaster en_US
dc.subject Huntingtin expression en_US
dc.subject Autophagy en_US
dc.title Effects of temporally controlled huntingtin expression and autophagy upregulation in drosophila melanogaster en_US
dc.type Thesis en_US
dc.type.qualificationlevel Master en_US
dc.type.qualificationname MS en_US
dc.publisher.department Evolutionary and Integrative Biology Unit (EIBU) en_US


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