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DC Field | Value | Language |
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dc.contributor.author | Meetei, Amom Ruhikanta | - |
dc.contributor.author | Rao, M R S | - |
dc.date.accessioned | 2011-04-01T09:52:01Z | - |
dc.date.available | 2011-04-01T09:52:01Z | - |
dc.date.issued | 2001-11-06 | - |
dc.identifier | 1064-3745 | en_US |
dc.identifier.citation | Methods in Molecular Biology, vol. 182: In Vitro Mutagenesis Protocols, 2nd ed. Edited by: J. Braman, Humana Press Inc., Totowa, NJ, 95-102 (2001) | en_US |
dc.identifier.uri | https://libjncir.jncasr.ac.in/xmlui/10572/102 | - |
dc.description | Restricted access. | en_US |
dc.description.abstract | Generating mutant proteins has become a routine strategy for molecular and structural biologists, to understand the structure-function relationship of a given protein of interest. In this, site-directed mutagenesis has proven to be a valuable technique. After the advent of polymerase chain reaction (PCR), PCR- mediated mutagenesis has been the method of choice to change from one amino acid to another, which has replaced the classic Kunkel’s method (1). | en_US |
dc.description.uri | http://dx.doi.org/10.1385/1-59259-194-9:095 | en_US |
dc.language.iso | en | en_US |
dc.publisher | Humana Press Inc. | en_US |
dc.rights | © 2001 Humana Press Inc. | en_US |
dc.subject | Multiple Site-Specific Mutations | en_US |
dc.subject | Polymerase Chain Reaction | en_US |
dc.title | Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction | en_US |
dc.type | Book chapter | en_US |
Appears in Collections: | Research Papers (M.R.S. Rao) |
Files in This Item:
File | Description | Size | Format | |
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sl.no.92.2001.Methods in Molecular Biology 182, 95-102..pdf Restricted Access | 147.02 kB | Adobe PDF | View/Open Request a copy |
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