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dc.contributor.authorAkhade, Vijay Suresh
dc.contributor.authorDighe, Shrinivas Nivrutti
dc.contributor.authorKataruka, Shubhangini
dc.contributor.authorRao, M. R. S.
dc.date.accessioned2017-01-24T06:22:39Z-
dc.date.available2017-01-24T06:22:39Z-
dc.date.issued2016
dc.identifier.citationAkhade, V. S.; Dighe, S. N.; Kataruka, S.; Rao, M. R. S., Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Research 2016, 44 (1), 387-401 http://dx.doi.org/10.1093/nar/gkv1023en_US
dc.identifier.citationNucleic Acids Researchen_US
dc.identifier.citation44en_US
dc.identifier.citation1en_US
dc.identifier.issn0305-1048
dc.identifier.urihttps://libjncir.jncasr.ac.in/xmlui/10572/2113-
dc.descriptionOpen Accessen_US
dc.description.abstractLong non coding RNAs (lncRNAs) have emerged as important regulators of various biological processes. LncRNAs also behave as response elements or targets of signaling pathway(s) mediating cellular function. Wnt signaling is important in regulating mammalian spermatogenesis. Mrhl RNA negatively regulates canonical Wnt pathway and gets down regulated upon Wnt signaling activation in mouse spermatogonial cells. Also, mrhl RNA regulates expression of genes pertaining to Wnt pathway and spermatogenesis by binding to chromatin. In the present study, we delineate the detailed molecular mechanism of Wnt signaling induced mrhl RNA down regulation in mouse spermatogonial cells. Mrhl RNA has an independent transcription unit and our various experiments like Chromatin Immunoprecipitation (in cell line as well as mouse testis) and shRNA mediated down regulation convincingly show that beta-catenin and TCF4, which are the key effector proteins of the Wnt signaling pathway are required for down regulation of mrhl RNA. We have identified Ctbp1 as the corepressor and its occupancy on mrhl RNA promoter depends on both beta-catenin and TCF4. Upon Wnt signaling activation, Ctbp1 mediated histone repression marks increase at the mrhl RNA promoter. We also demonstrate that Wnt signaling induced mrhl RNA down regulation results in an up regulation of various meiotic differentiation marker genes.en_US
dc.description.uri1362-4962en_US
dc.description.urihttp://dx.doi.org/10.1093/nar/gkv1023en_US
dc.language.isoEnglishen_US
dc.publisherOxford University Pressen_US
dc.rights@Oxford University Press, 2016en_US
dc.subjectBiochemistry & Molecular Biologyen_US
dc.subjectBeta-Cateninen_US
dc.subjectTranscriptional Activationen_US
dc.subjectSeminiferous Epitheliumen_US
dc.subjectTarget Genesen_US
dc.subjectChromatinen_US
dc.subjectDifferentiationen_US
dc.subjectSpermatogenesisen_US
dc.subjectRecruitmenten_US
dc.subjectExpressionen_US
dc.subjectProteinen_US
dc.titleMechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cellsen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (M.R.S. Rao)

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