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https://libjncir.jncasr.ac.in/xmlui/handle/10572/2189| Title: | Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On |
| Authors: | Shetty, Deeti K. Shetty, Ronak Inamdar, Maneesha S. |
| Keywords: | Cell Biology Biotechnology & Applied Microbiology |
| Issue Date: | 2016 |
| Publisher: | Elsevier Science Bv |
| Citation: | Shetty, D. K.; Shetty, R.; Inamdar, M. S., Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On. Stem Cell Research 2016, 16 (2), 246-248 http://dx.doi.org/10.1016/j.scr.2015.12.045 Stem Cell Research 16 2 |
| Abstract: | Human embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On cells can be used for inducible expression of any coding sequence cloned into pTRE-Tight vector. For example, in human embryonic stem cells, Tet-On system has been used to generate SOX2 overexpression cell line (Adachi et al., 2010). (C) 2016 The Authors. Published by Elsevier B.V. |
| Description: | Restricted Access |
| URI: | https://libjncir.jncasr.ac.in/xmlui/10572/2189 |
| ISSN: | 1873-5061 |
| Appears in Collections: | Research Papers (Maneesha S. Inamdar) |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 251.pdf Restricted Access | 437.47 kB | Adobe PDF | View/Open Request a copy |
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