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https://libjncir.jncasr.ac.in/xmlui/handle/10572/2190| Title: | Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR |
| Authors: | Shetty, Ronak Inamdar, Maneesha S. |
| Keywords: | Cell Biology Biotechnology & Applied Microbiology |
| Issue Date: | 2016 |
| Publisher: | Elsevier Science Bv |
| Citation: | Shetty, R.; Inamdar, M. S., Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR. Stem Cell Research 2016, 16 (2), 271-273 http://dx.doi.org/10.1016/j.scr.2015.12.043 Stem Cell Research 16 2 |
| Abstract: | Human embryonic stem cell line BJNhem20-TetR was generated using non-viral method. The construct pCAG-TetRnls was transfected using microporation procedure. BJNhem20-TetR can subsequently be transfected with any vector harbouring a TetO (Tet operator) sequence to generate doxycycline based inducible line. For example, in human embryonic stem cells, the pSuperior based TetO system has been transfected into a TetR containing line to generate OCT4 knockdown cell line (Zafarana et al., 2009). Thus BJNhem20-TetR can be used as a tool to perturb gene expression in human embryonic stem cells. (C) 2016 The Authors. Published by Elsevier B.V. |
| Description: | Restricted Access |
| URI: | https://libjncir.jncasr.ac.in/xmlui/10572/2190 |
| ISSN: | 1873-5061 |
| Appears in Collections: | Research Papers (Maneesha S. Inamdar) |
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|---|---|---|---|---|
| 252.pdf Restricted Access | 536.22 kB | Adobe PDF | View/Open Request a copy |
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