Please use this identifier to cite or link to this item: https://libjncir.jncasr.ac.in/xmlui/handle/10572/428
Full metadata record
DC FieldValueLanguage
dc.contributor.authorUnni, Emmanual-
dc.contributor.authorRao, M R S-
dc.date.accessioned2012-02-15T08:52:23Z-
dc.date.available2012-02-15T08:52:23Z-
dc.date.issued1985-06-
dc.identifier0250-5991en_US
dc.identifier.citationJournal of Biosciences 7(3-4), 345-357 (1985)en_US
dc.identifier.urihttps://libjncir.jncasr.ac.in/xmlui/10572/428-
dc.descriptionRestricted Accessen_US
dc.description.abstractA quantitative analysis of the different types of germ cells present in the seminiferous tubules of vitamin A-deficient-retinoate maintained rats revealed that the number of pachytene spermatocytes and spermatogonia was greatly reduced in the deficient rats. Spermatids were virtually absent in the deficient tubules which contained mostly spermatogonia and preleptotene spermatocytes along with the Sertoli cells. There was no change in the number of Sertoli cells present in the tubules of deficient rats as compared to that of normal rats. Following supplementation of retinyl acetate to vitamin A-deficient-retinoate maintained rats, there was an immediate thinning of the germinal epithelium resulting from the sloughing off of the damaged spermatocytes which were beyond repair. However, after 12 days of vitamin A supplementation fresh batch of pachytene spermatocytes started appearing while by day 16 round spermatids could be seen. Analysis of the acid soluble proteins from nuclei on different types of Polyacrylamide gel electrophoretic systems has revealed that the levels of the testis specific histone variants Hlt, TH2A and TH2B, synthesized predominantly in the pachytene spermatocytes were greatly reduced in the testes of retinoate maintained rats. Following supplementation of retinyl acetate for either 4 days or 8 days the levels of these histone variants further decreased which correlated with the decrease in the number of pachytene spermatocytes. However, by day 12 of supplementation onwards, their levels started increasing and reached near normal levels by day 24 of vitamin A-supplementation.en_US
dc.description.urihttp://dx.doi.org/10.1007/BF02716796en_US
dc.language.isoenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.rights© 1985 Indian Academy of Sciencesen_US
dc.subjectSpermatogenesisen_US
dc.subjectgerm cellsen_US
dc.subjecthistone variantsen_US
dc.subjectvitamin A deficiency and repletionen_US
dc.titleA quantitative analysis of germ cells and the histone variants in the testes of vitamin A-deficient rats and during subsequent repletion with vitamin Aen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (M.R.S. Rao)

Files in This Item:
File Description SizeFormat 
sl.no.47.1985.J. Biosci., Vol. 7, 3 & 4, 345-357.pdf
  Restricted Access
2.27 MBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.