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Title: A Model for the Regulation of delta-Aminolaevulinate Synthetase Induction in Rat Liver
Authors: Padmanaban, Govindarajan
Rao, M R S
Malathi, Krishnamachari
Keywords: 5-Aminolevulinate Synthetase -metabolism
Allylisopropylacetamide- pharmacology
Carbon Radioisotopes
Citrates - metabolism
Cytochrome P-450 Enzyme System -metabolism
Dactinomycin - pharmacology
Enzyme Induction
Heme - metabolism
Hydrocortisone - pharmacology
Iron Radioisotopes
Liver - enzymology
Liver - metabolism
Phenobarbital - pharmacology
Protein Binding
Issue Date: Aug-1973
Publisher: The Biochemical Society
Citation: Biochemical Journal 134(4), 847–857 (1973)
Abstract: A reciprocal relationship exists between the cytochrome P-450 content and δ-aminolaevulinate synthetase activity in adult rats. In young rats the basal δ-aminolaevulinate synthetase activity is higher and the cytochrome P-450 content is lower compared with the adult rat liver. Administration of allylisopropylacetamide neither induces the enzyme nor causes degradation of cytochrome P-450 in the young rat liver, unlike adult rat liver. Allylisopropylacetamide fails to induce δ-aminolaevulinate synthetase in adrenalectomized–ovariectomized animals or intact animals pretreated with successive doses of the drug, in the absence of cortisol. The cortisol-mediated induction of the enzyme is sensitive to actinomycin D. Allylisopropylacetamide administration degrades microsomal haem but not nuclear haem. Haem does not counteract the decrease in cytochrome P-450 content caused by allylisopropylacetamide administration, but there is evidence for the formation of drug-resistant protein-bound haem in liver microsomal material under these conditions. Phenobarbital induces δ-aminolaevulinate synthetase under conditions when there is no breakdown of cytochrome P-450. On the basis of these results and those already published, a model is proposed for the regulation of δ-aminolaevulinate synthetase induction in rat liver.
Description: Restricted Access
Other Identifiers: 0264-6021
Appears in Collections:Research Papers (M.R.S. Rao)

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