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Title: Localization of testis-variant histones in rat testis chromatin
Authors: Rao, M R S
Rao, Basuthkar Jagadeeshwar
Ganguly, Jagannath
Keywords: Animals
Chromatography Gel
DNA - metabolism
Polyacrylamide Gel
Histones - metabolism
Nucleosomes - metabolism
Testis - metabolism
Issue Date: 1982
Publisher: The Biochemical Society
Citation: Biochemical Journal 205(1), 15-21 (1982)
Abstract: Nucleosome core particles and oligonucleosomes were isolated by digesting rat testis nuclei with micrococcal nuclease to 20% acid-solubility, followed by fractionation of the digest on a Bio-Gel A-5m column. The core particles thus isolated were characterized on the basis of their DNA length of 151 +/- 5 base-pairs and sedimentation coefficient of 11.4S. Analysis of the acid-soluble proteins of the core particles indicated that histones TH2B and X2 are constituents of the core particles, in addition to the somatic histones H2A, H2B, H3 and H4. The acid-soluble proteins of the oligonucleosomes comprised all the histones, including both the somatic (H1, H2A, H2B, H3, H4 and X2) and the testis-specific ones (TH1 and TH2B). It was also observed that histones TH1 and H1 are absent from the core particles and were readily extracted from the chromatin by 0.6 M-NaCl, which indicated that both of them are bound to the linker DNA.
Description: Restricted Access
Other Identifiers: 0264-6021
Appears in Collections:Research Papers (M.R.S. Rao)

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