Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction

dc.contributor.author	Meetei, Amom Ruhikanta
dc.contributor.author	Rao, M R S
dc.date.accessioned	2011-04-01T09:52:01Z
dc.date.available	2011-04-01T09:52:01Z
dc.date.issued	2001-11-06
dc.identifier	1064-3745	en_US
dc.identifier.citation	Methods in Molecular Biology, vol. 182: In Vitro Mutagenesis Protocols, 2nd ed. Edited by: J. Braman, Humana Press Inc., Totowa, NJ, 95-102 (2001)	en_US
dc.identifier.uri	https://libjncir.jncasr.ac.in/xmlui/10572/102
dc.description	Restricted access.	en_US
dc.description.abstract	Generating mutant proteins has become a routine strategy for molecular and structural biologists, to understand the structure-function relationship of a given protein of interest. In this, site-directed mutagenesis has proven to be a valuable technique. After the advent of polymerase chain reaction (PCR), PCR- mediated mutagenesis has been the method of choice to change from one amino acid to another, which has replaced the classic Kunkel’s method (1).	en_US
dc.description.uri	http://dx.doi.org/10.1385/1-59259-194-9:095	en_US
dc.language.iso	en	en_US
dc.publisher	Humana Press Inc.	en_US
dc.rights	© 2001 Humana Press Inc.	en_US
dc.subject	Multiple Site-Specific Mutations	en_US
dc.subject	Polymerase Chain Reaction	en_US
dc.title	Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction	en_US
dc.type	Book chapter	en_US