Faculty publications (MBGU): Recent submissions

  • Mycobacterium tuberculosis Rho Is an NTPase with Distinct Kinetic Properties and a Novel RNA-Binding Subdomain 
    Mitra, Anirban; Misquitta, Rachel; Nagaraja, V. (Public Library of Science, 2014)
    Two mechanisms - factor independent and dependent termination - ensure the completion of RNA synthesis in eubacteria. Factor-dependent mechanism relies on the Rho protein to terminate transcription by interacting with RNA ...
  • Conditional silencing of topoisomerase I gene of Mycobacterium tuberculosis validates its essentiality for cell survival 
    Ahmed, Wareed; Menon, Shruti; Godbole, Adwait Anand; Karthik, Pullela V. D. N. B.; Nagaraja, V. (Oxford University Press, 2014)
    Topoisomerases are an important class of enzymes for regulating the DNA transaction processes. Mycobacterium tuberculosis (Mtb) is one of the most formidable pathogens also posing serious challenges for therapeutic ...
  • Direct regulation of topoisomerase activity by a nucleoid-associated protein 
    Ghosh, Soumitra; Mallick, Bratati; Nagaraja, V. (Oxford University Press, 2014)
    The topological homeostasis of bacterial chromosomes is maintained by the balance between compaction and the topological organization of genomes. Two classes of proteins play major roles in chromosome organization: the ...
  • Molecular Basis for the Differential Quinolone Susceptibility of Mycobacterial DNA Gyrase 
    Kumar, Rupesh; Madhumathi, Bhavani Shankar; Nagaraja, V. (American Society Microbiology, 2014)
    DNA gyrase is a type II topoisomerase that catalyzes the introduction of negative supercoils in the genomes of eubacteria. Fluoroquinolones (FQs), successful as drugs clinically, target the enzyme to trap the gyrase-DNA ...
  • Allosteric Transition Induced by Mg2+ Ion in a Transactivator Monitored by SERS 
    Kundu, Partha P.; Bhowmick, Tuhin; Swapna, Ganduri; Kumar, G. V. Pavan; Nagaraja, V.; Narayana, Chandrabhas (American Chemical Society, 2014)
    We demonstrate the utility of the surface-enhanced Raman spectroscopy (SERS) to monitor conformational transitions in protein upon ligand binding. The changes in protein's secondary and tertiary structures were monitored ...
  • Inhibition of Mycobacterium tuberculosis topoisomerase I by m-AMSA, a eukaryotic type II topoisomerase poison 
    Godbole, Adwait Anand; Ahmed, Wareed; Bhat, Rajeshwari Subray; Bradley, Erin K.; Ekins, Sean; Nagaraja, V. (Academic Press Inc Elsevier Science, 2014)
    m-AMSA, an established inhibitor of eukaryotic type II topoisomerases, exerts its cidal effect by binding to the enzyme-DNA complex thus inhibiting the DNA religation step. The molecule and its analogues have been successfully ...
  • Targeting Mycobacterium tuberculosis nucleoid-associated protein HU with structure-based inhibitors 
    Bhowmick, Tuhin; Ghosh, Soumitra; Dixit, Karuna; Ganesan, Varsha; Ramagopal, Udupi A.; Dey, Debayan; Sarma, Siddhartha P.; Ramakumar, Suryanarayanarao; Nagaraja, V. (Nature Publishing Group, 2014)
    The nucleoid-associated protein HU plays an important role in maintenance of chromosomal architecture and in global regulation of DNA transactions in bacteria. Although HU is essential for growth in Mycobacterium tuberculosis ...
  • Synthesis of Hybrid Cyclic Peptoids and Identification of Autophagy Enhancer 
    Rajasekhar, Kolla; Narayanaswamy, Nagarjun; Mishra, Piyush; Suresh, S. N.; Manjithaya, Ravi; Govindaraju, T. (Wiley-V C H Verlag Gmbh, 2014)
    Cyclic peptoids are potential candidates for diverse biological activities. However, applications of cyclic peptoids are limited by the synthetic difficulties, conformational flexibility of large cyclic peptoids, and lack ...
  • A probe for ratiometric near-infrared fluorescence and colorimetric hydrogen sulfide detection and imaging in live cells 
    Maity, Debabrata; Raj, Anand; Samanta, Pralok K.; Karthigeyan, D.; Kundu, Tapas Kumar; Pati, Swapan Kumar; Govindaraju, T. (Royal Society of Chemistry, 2014)
    A ratiometric, near-infrared (NIR), fluorescence and colorimetric probe DNPOCy for hydrogen sulfide (H2S) has been developed. The chemical basis for the operation of the probe is thiolysis of a dinitrophenyl ether, which ...
  • Temperature sensitivity of circadian clocks is conserved across Drosophila species melanogaster, malerkotliana and ananassae 
    Prabhakaran, Priya M.; Sheeba, Vasu (Informa Healthcare, 2014)
    Light and temperature are the major environmental cycles that can synchronize circadian rhythms in a variety of organisms. Previously, we have shown that under light/dark cycles of various photoperiods, the Drosophila ...
  • Simulating natural light and temperature cycles in the laboratory reveals differential effects on activity/rest rhythm of four Drosophilids 
    Prabhakaran, Priya M.; Sheeba, Vasu (Springer, 2014)
    Recent studies under semi-natural conditions have revealed various unique features of activity/rest rhythms in Drosophilids that differ from those under standard laboratory conditions. An additional afternoon peak (A-peak) ...
  • The Grafting of Universal T-Helper Epitopes Enhances Immunogenicity of HIV-1 Tat Concurrently Improving Its Safety Profile 
    Kashi, Venkatesh P.; Jacob, Rajesh A.; Shamanna, Raghavendra A.; Menon, Malini; Balasiddaiah, Anangi; Varghese, Rebu K.; Bachu, Mahesh; Ranga, Udaykumar (Public Library of Science, 2014)
    Extracellular Tat (eTat) plays an important role in HIV-1 pathogenesis. The presence of anti-Tat antibodies is negatively correlated with disease progression, hence making Tat a potential vaccine candidate. The cytotoxicity ...
  • Reactivation of latent HIV-1 by new semi-synthetic ingenol esters 
    Jose, Diego Pandelo; Bartholomeeusen, Koen; da Cunha, Rodrigo Delvecchio; Abreu, Celina Monteiro; Glinski, Jan; Ferreira da Costa, Thais Barbizan; Mello Bacchi Rabay, Ana Flavia; Pianowski Filho, Luiz Francisco; Dudycz, Lech W.; Ranga, Udaykumar; Peterlin, Boris Matija; Pianowski, Luiz Francisco; Tanuri, Amilcar; Aguiar, Renato Santana (Academic Press Inc Elsevier Science, 2014)
    The ability of HIV to establish long-lived latent infection is mainly due to transcriptional silencing of viral genome in resting memory T lymphocytes. Here, we show that new semi-synthetic ingenol esters reactivate latent ...
  • Evaluation of chitosan nanoformulations as potent anti-HIV therapeutic systems 
    Ramana, Lakshmi Narashimhan; Sharma, Shilpee; Sethuraman, Swaminathan; Ranga, Udaykumar; Krishnan, Uma Maheswari (Elsevier Science Bv, 2014)
    Background: Antiretroviral Therapy (ART) is currently the major therapeutic intervention in the treatment of AIDS. ART, however, is severely limited due to poor availability, high cytotoxicity, and enhanced metabolism and ...
  • Genetic Diversity in HIV-1 Subtype C LTR from Brazil and Mozambique Generates New Transcription Factor-Binding Sites 
    Boullosa, Jose; Bachu, Mahesh; Bila, Dulce; Ranga, Udaykumar; Sueffert, Theodoro; Sasazawa, Tomoko; Tanuri, Amilcar (Mdpi Ag, 2014)
    The HIV-1 subtype C has been substituting the subtype B population in southern Brazil. This phenomenon has been previously described in other countries, suggesting that subtype C may possess greater fitness than other ...
  • The dimerization domain of PfCENP-C is required for its functions as a centromere protein in human malaria parasite Plasmodium falciparum 
    Verma, Garima; Surolia, Namita (Biomed Central Ltd, 2014)
    Background: The conserved centromere-associated proteins, CENH3 (or CENP-A) and CENP-C are indispensable for the functional centromere-kinetochore assembly, chromosome segregation, cell cycle progression, and viability. ...
  • ARF1-GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis 
    Khadilkar, Rohan J.; Rodrigues, Diana; Mote, Ridim Dadasaheb; Sinha, Arghyashree Roychowdhury; Kulkarni, Vani; Magadi, Srivathsa Subramanya; Inamdar, Maneesha S. (National Academy of Sciences, 2014)
    Drosophila melanogaster larval hematopoiesis is a well-established model to study mechanisms that regulate hematopoietic niche maintenance and control of blood cell precursor (prohemocyte) differentiation. Molecules that ...
  • Rad51-Rad52 Mediated Maintenance of Centromeric Chromatin in Candida albicans 
    Mitra, Sreyoshi; Gomez-Raja, Jonathan; Larriba, German; Dubey, Dharani Dhar; Sanyal, Kaustuv (Public Library of Science, 2014)
    Specification of the centromere location in most eukaryotes is not solely dependent on the DNA sequence. However, the non-genetic determinants of centromere identity are not clearly defined. While multiple mechanisms, ...
  • Analysis of the Genome and Transcriptome of Cryptococcus neoformans var. grubii Reveals Complex RNA Expression and Microevolution Leading to Virulence Attenuation 
    Janbon, Guilhem; Ormerod, Kate L.; Paulet, Damien; Byrnes, Edmond J., III; Yadav, Vikas; Chatterjee, Gautam; Mullapudi, Nandita; Hon, Chung-Chau; Billmyre, R. Blake; Brunel, Francois; Bahn, Yong-Sun; Chen, Weidong; Chen, Yuan; Chow, Eve W. L.; Coppee, Jean-Yves; Floyd-Averette, Anna; Gaillardin, Claude; Gerik, Kimberly J.; Goldberg, Jonathan; Gonzalez-Hilarion, Sara; Gujja, Sharvari; Hamlin, Joyce L.; Hsueh, Yen-Ping; Ianiri, Giuseppe; Jones, Steven; Kodira, Chinnappa D.; Kozubowski, Lukasz; Lam, Woei; Marra, Marco; Mesner, Larry D.; Mieczkowski, Piotr A.; Moyrand, Frederique; Nielsen, Kirsten; Proux, Caroline; Rossignol, Tristan; Schein, Jacqueline E.; Sun, Sheng; Wollschlaeger, Carolin; Wood, Ian A.; Zeng, Qiandong; Neuveglise, Cecile; Newlon, Carol S.; Perfect, John R.; Lodge, Jennifer K.; Idnurm, Alexander; Stajich, Jason E.; Kronstad, James W.; Sanyal, Kaustuv; Heitman, Joseph; Fraser, James A.; Cuomo, Christina A.; Dietrich, Fred S. (Public Library of Science, 2014)
    Cryptococcus neoformans is a pathogenic basidiomycetous yeast responsible for more than 600,000 deaths each year. It occurs as two serotypes (A and D) representing two varieties (i.e. grubii and neoformans, respectively). ...
  • Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila 
    Srinivasan, Bharath; Forouhar, Farhad; Shukla, Arpit; Sampangi, Chethana; Kulkarni, Sonia; Abashidze, Mariam; Seetharaman, Jayaraman; Lew, Scott; Mao, Lei; Acton, Thomas B.; Xiao, Rong; Everett, John K.; Montelione, Gaetano T.; Tong, Liang; Balaram, Hemalatha (Wiley-Blackwell, 2014)
    Cytosolic nucleotidase II (cN-II) from Legionellapneumophila (Lp) catalyzes the hydrolysis of GMP and dGMP displaying sigmoidal curves, whereas catalysis of IMP hydrolysis displayed a biphasic curve in the initial rate ...

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