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Mapping of post-translational modifications of spermatid-specific linker histone H1-like protein, HILS1

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dc.contributor.author Mishra, Laxmi N.
dc.contributor.author Gupta, Nikhil
dc.contributor.author Rao, M. R. S.
dc.date.accessioned 2016-10-28T06:00:53Z
dc.date.available 2016-10-28T06:00:53Z
dc.date.issued 2015
dc.identifier.citation Journal of Proteomics en_US
dc.identifier.citation 128 en_US
dc.identifier.citation Mishra, L. N.; Gupta, N.; Rao, S. M. R., Mapping of post-translational modifications of spermatid-specific linker histone H1-like protein, HILS1. Journal of Proteomics 2015, 128, 218-230. en_US
dc.identifier.issn 1874-3919
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/1928
dc.description Restricted access en_US
dc.description.abstract In mammalian spermiogenesis, haploid round spermatids undergo dramatic biochemical and morphological changes and transform into motile mature spermatozoa. A majority of the histones are replaced by transition proteins during mid-spermiogenesis and later replaced by protamines, which occupy the sperm chromatin. In mammals, 11 linker histone H1 subtypes have been reported. Among them, Hit, HILS1, and H1T2 are uniquely expressed in testis, with the expression of HILS1 and H1T2 restricted to spermiogenesis. However, there is a lack of knowledge about linker histone role in the nuclear reorganization during mammalian spermiogenesis. Here, we report a method for separation of endogenous HILS1 protein from other rat testis linker histones by reversed-phase high-performance liquid chromatography (RP-HPLC) and identification of 15 novel post-translational modifications of HILS1, which include lysine acetylation and serine/threonine/tyrosine phosphorylation sites. Immunofluorescence studies demonstrate the presence of linker histone HILS1 and HILS1Y78p during different steps of spermiogenesis from early elongating to condensing spermatids. (C) 2015 Elsevier B.V. All rights reserved. en_US
dc.description.uri 1876-7737 en_US
dc.description.uri http://dx.doi.org/10.1016/j.jprot.2015.08.001 en_US
dc.language.iso English en_US
dc.publisher Elsevier Science Bv en_US
dc.rights ?Elsevier Science Bv, 2015 en_US
dc.subject Biochemical Research Methods en_US
dc.subject Linker histones en_US
dc.subject HILS1 en_US
dc.subject Mass spectrometry en_US
dc.subject Post-translational modifications en_US
dc.subject Chromatin remodeling en_US
dc.subject Spermiogenesis en_US
dc.subject C-Terminal Domain en_US
dc.subject Haploid Germ-Cells en_US
dc.subject Globular Domain en_US
dc.subject Mammalian Spermiogenesis en_US
dc.subject Tyrosine Phosphorylation en_US
dc.subject Mass-Spectrometry en_US
dc.subject DNA Condensation en_US
dc.subject H1 Variants en_US
dc.subject Chromatin Condensation en_US
dc.subject Nucleosome Binding en_US
dc.title Mapping of post-translational modifications of spermatid-specific linker histone H1-like protein, HILS1 en_US
dc.type Article en_US


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