Mitra, Anirban; Misquitta, Rachel; Nagaraja, V.(Public Library of Science, 2014)
Two mechanisms - factor independent and dependent termination - ensure the completion of RNA synthesis in eubacteria. Factor-dependent mechanism relies on the Rho protein to terminate transcription by interacting with RNA ...
Ahmed, Wareed; Menon, Shruti; Godbole, Adwait Anand; Karthik, Pullela V. D. N. B.; Nagaraja, V.(Oxford University Press, 2014)
Topoisomerases are an important class of enzymes for regulating the DNA transaction processes. Mycobacterium tuberculosis (Mtb) is one of the most formidable pathogens also posing serious challenges for therapeutic ...
Ghosh, Soumitra; Mallick, Bratati; Nagaraja, V.(Oxford University Press, 2014)
The topological homeostasis of bacterial chromosomes is maintained by the balance between compaction and the topological organization of genomes. Two classes of proteins play major roles in chromosome organization: the ...
Kumar, Rupesh; Madhumathi, Bhavani Shankar; Nagaraja, V.(American Society Microbiology, 2014)
DNA gyrase is a type II topoisomerase that catalyzes the introduction of negative supercoils in the genomes of eubacteria. Fluoroquinolones (FQs), successful as drugs clinically, target the enzyme to trap the gyrase-DNA ...
Kundu, Partha P.; Bhowmick, Tuhin; Swapna, Ganduri; Kumar, G. V. Pavan; Nagaraja, V.; Narayana, Chandrabhas(American Chemical Society, 2014)
We demonstrate the utility of the surface-enhanced Raman spectroscopy (SERS) to monitor conformational transitions in protein upon ligand binding. The changes in protein's secondary and tertiary structures were monitored ...
m-AMSA, an established inhibitor of eukaryotic type II topoisomerases, exerts its cidal effect by binding to the enzyme-DNA complex thus inhibiting the DNA religation step. The molecule and its analogues have been successfully ...
The nucleoid-associated protein HU plays an important role in maintenance of chromosomal architecture and in global regulation of DNA transactions in bacteria. Although HU is essential for growth in Mycobacterium tuberculosis ...
Ahmed, Wareed; Menon, Shruti; Karthik, Pullela V. D. N. B.; Nagaraja, Valakunja(Oxford University Press, 2016)
The opposing catalytic activities of topoisomerase I (TopoI/relaxase) and DNA gyrase (supercoiling enzyme) ensure homeostatic maintenance of bacterial chromosome supercoiling. Earlier studies in Es-cherichia coli suggested ...
As a human fungal pathogen, Candida albicans can cause a wide variety of disease conditions ranging from superficial to systemic infections. Many of these infections are caused by an inherent ability of the pathogen to ...