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Surface-Functionalized Silk Fibroin Films as a Platform To Guide Neuron-like Differentiation of Human Mesenchymal Stem Cells

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dc.contributor.author Manchineella, Shivaprasad
dc.contributor.author Thrivikraman, Greeshma
dc.contributor.author Basu, Bikramjit
dc.contributor.author Govindaraju, T.
dc.date.accessioned 2017-01-24T06:35:04Z
dc.date.available 2017-01-24T06:35:04Z
dc.date.issued 2016
dc.identifier.citation Manchineella, S.; Thrivikraman, G.; Basu, B.; Govindaraju, T., Surface-Functionalized Silk Fibroin Films as a Platform To Guide Neuron-like Differentiation of Human Mesenchymal Stem Cells. Acs Applied Materials & Interfaces 2016, 8 (35), 22849-22859 http://dx.doi.org/10.1021/acsami.6b06403 en_US
dc.identifier.citation ACS Applied Materials & Interfaces en_US
dc.identifier.citation 8 en_US
dc.identifier.citation 35 en_US
dc.identifier.issn 1944-8244
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/2197
dc.description Restricted Access en_US
dc.description.abstract Surface interactions at the biomaterial cellular interface determine the proliferation and differentiation of stem cells. Manipulating such interactions through the surface chemistry of scaffolds renders control over directed stem cell differentiation into the cell lineage of interest. This approach is of central importance for stem cell-based tissue engineering and regenerative therapy applications. In the present study, silk fibroin films (SFFs) decorated with integrin-binding laminin peptide motifs (YIGSR and GYIGSR) were prepared and employed for in vitro adult stem cell-based neural tissue engineering applications. Functionalization of SFFs with short peptides showcased the peptide sequence and nature of functionalization-dependent differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs). Intriguingly, covalently functionalized SFFs with GYIGSR hexapeptide (CL2-SFF) supported hMSC proliferation and maintenance in an undifferentiated pluripotent state and directed the differentiation of hMSCs into neuron-like cells in the presence of a biochemical cue, on-demand. The observed morphological changes were further corroborated by the up-regulation of neuronal-specific marker gene expression (MAP2, TUBB3, NEFL), confirmed through semiquantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The enhanced proliferation and on-demand directed differentiation of adult stem cells (hMSCs) by the use of an economically viable short recognition peptide (GYIGSR), as opposed to the integrin recognition protein laminin, establishes the potential of SFFs for neural tissue engineering and regenerative therapy applications. en_US
dc.description.uri http://dx.doi.org/10.1021/acsami.6b06403 en_US
dc.language.iso English en_US
dc.publisher American Chemical Society en_US
dc.rights @American Chemical Society, 2016 en_US
dc.subject Materials Science en_US
dc.subject silk fibroin en_US
dc.subject laminin peptide en_US
dc.subject surface functionalization en_US
dc.subject biomaterials en_US
dc.subject tissue engineering en_US
dc.subject transdifferentiation en_US
dc.subject neuron-like stem cells en_US
dc.subject Extracellular-Matrix en_US
dc.subject In-Vitro en_US
dc.subject Regenerative Medicine en_US
dc.subject Growth-Factors en_US
dc.subject Retinoic-Acid en_US
dc.subject Tissue en_US
dc.subject Biomaterials en_US
dc.subject Fate en_US
dc.subject Nanofibers en_US
dc.subject Substrate en_US
dc.title Surface-Functionalized Silk Fibroin Films as a Platform To Guide Neuron-like Differentiation of Human Mesenchymal Stem Cells en_US
dc.type Article en_US


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