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Stage-Dependent Changes In Localization Of A Germ Cell-Specific Lamin During Mammalian Spermatogenesis

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dc.contributor.author Sudhakar, L
dc.contributor.author Rao, M R S
dc.date.accessioned 2012-02-15T06:38:37Z
dc.date.available 2012-02-15T06:38:37Z
dc.date.issued 1990-12-25
dc.identifier 0021-9258 en_US
dc.identifier.citation Journal of Biological Chemistry 265, 22526-22532 (1990) en_US
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/422
dc.description Restricted Access en_US
dc.description.abstract We had earlier identified a 110/120-kDa protein specific to nuclear matrix of rat pachytene spermatocytes (Behal, A., Prakash, K., and Rao, M. R. S. (1987) J. Biol. Chem. 262, 10898-10902). This protein is now shown to be a disulfide-linked homodimer of a 60-kDa polypeptide. Indirect immunofluorescence and Western blot analyses using anti-120-kDa polyclonal antibodies have shown that this protein is a component of the pore-complex lamina structure of spermatogonia. As germ cells enter meiotic prophase and the lamina structure disassembles, this polypeptide is redistributed in the nucleus and can be isolated as a component of synaptonemal complexes. Following meiotic division, this 60-kDa protein is relocalized in the lamina, then representing the sole major component of the lamina structure of round spermatids. The identity of the 60-kDa protein in the pore-complex lamina fraction and synaptonemal complexes was further confirmed by two-dimensional analysis of iodinated tryptic peptides. Such an analysis has also shown that the germ cell-specific 60-kDa protein is related but not identical to somatic lamin B. en_US
dc.description.uri http://www.ncbi.nlm.nih.gov/pubmed/2266143 en_US
dc.description.uri http://www.jbc.org/content/265/36/22526.long en_US
dc.description.uri http://hwmaint.jbc.org/cgi/content/abstract/265/36/22526 en_US
dc.language.iso en en_US
dc.publisher American Society for Biochemistry and Molecular Biology Inc en_US
dc.rights © 1990 The American Society for Biochemistry and Molecular Biology Inc en_US
dc.subject Nuclear Lamina en_US
dc.subject Differential Expression en_US
dc.subject Synaptonemal Complexes en_US
dc.subject Xenopus-Laevis en_US
dc.subject Mevalonic Acid en_US
dc.subject Proteins en_US
dc.subject Rat en_US
dc.subject Phosphorylation en_US
dc.subject Disappearance en_US
dc.subject Interphase en_US
dc.title Stage-Dependent Changes In Localization Of A Germ Cell-Specific Lamin During Mammalian Spermatogenesis en_US
dc.type Article en_US


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