dc.contributor.author |
Sudhakar, L
|
|
dc.contributor.author |
Rao, M R S
|
|
dc.date.accessioned |
2012-02-15T06:38:37Z |
|
dc.date.available |
2012-02-15T06:38:37Z |
|
dc.date.issued |
1990-12-25 |
|
dc.identifier |
0021-9258 |
en_US |
dc.identifier.citation |
Journal of Biological Chemistry 265, 22526-22532 (1990) |
en_US |
dc.identifier.uri |
https://libjncir.jncasr.ac.in/xmlui/10572/422 |
|
dc.description |
Restricted Access |
en_US |
dc.description.abstract |
We had earlier identified a 110/120-kDa protein specific to nuclear matrix of rat pachytene spermatocytes (Behal, A., Prakash, K., and Rao, M. R. S. (1987) J. Biol. Chem. 262, 10898-10902). This protein is now shown to be a disulfide-linked homodimer of a 60-kDa polypeptide. Indirect immunofluorescence and Western blot analyses using anti-120-kDa polyclonal antibodies have shown that this protein is a component of the pore-complex lamina structure of spermatogonia. As germ cells enter meiotic prophase and the lamina structure disassembles, this polypeptide is redistributed in the nucleus and can be isolated as a component of synaptonemal complexes. Following meiotic division, this 60-kDa protein is relocalized in the lamina, then representing the sole major component of the lamina structure of round spermatids. The identity of the 60-kDa protein in the pore-complex lamina fraction and synaptonemal complexes was further confirmed by two-dimensional analysis of iodinated tryptic peptides. Such an analysis has also shown that the germ cell-specific 60-kDa protein is related but not identical to somatic lamin B. |
en_US |
dc.description.uri |
http://www.ncbi.nlm.nih.gov/pubmed/2266143 |
en_US |
dc.description.uri |
http://www.jbc.org/content/265/36/22526.long |
en_US |
dc.description.uri |
http://hwmaint.jbc.org/cgi/content/abstract/265/36/22526 |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
American Society for Biochemistry and Molecular Biology Inc |
en_US |
dc.rights |
© 1990 The American Society for Biochemistry and Molecular Biology Inc |
en_US |
dc.subject |
Nuclear Lamina |
en_US |
dc.subject |
Differential Expression |
en_US |
dc.subject |
Synaptonemal Complexes |
en_US |
dc.subject |
Xenopus-Laevis |
en_US |
dc.subject |
Mevalonic Acid |
en_US |
dc.subject |
Proteins |
en_US |
dc.subject |
Rat |
en_US |
dc.subject |
Phosphorylation |
en_US |
dc.subject |
Disappearance |
en_US |
dc.subject |
Interphase |
en_US |
dc.title |
Stage-Dependent Changes In Localization Of A Germ Cell-Specific Lamin During Mammalian Spermatogenesis |
en_US |
dc.type |
Article |
en_US |