Single-stranded DNA binding proteins (SSBs) are known to play a role in DNA replication and recombination in prokaryotes. An SSB was previously purified from the yeast Saccharomyces cerevisiae. This SSB stimulated the activity of a cognate strand exchange protein (SEP1) in vitro suggesting a role in recombination. We have cloned and functionally analyzed the gene encoding this protein. DNA sequencing of the cloned DNA revealed a 621 amino acid open reading frame with a coding potential for a Mr 70,269 polypeptide. Highly significant amino acid homology was detected between this S.cerevisiae gene and the Mr 70,000 subunit polypeptide of human RP-A, a cellular protein essential for SV40 DNA replication in vitro. Therefore, we named the S.cerevisiae gene RPA1. RPA1 encodes an essential function in this organism as shown by tetrad analysis of heterozygous insertion mutants and is continuously required for mitotic growth. Cells lacking RPA1 accumulate as multiply budded cells with a single nucleus suggesting a defect in DNA replication.
Fishel, Richard; Lescoe, Mary Kay; Rao, M R S; Copeland, Neal G; Jenkins, Nancy A; Garber, Judy; Kane, Michael; Kolodner, Richard(Cell Press, 1993-12)
We have identified a human homolog of the bacterial
MutS and S. cerevisiae MSH proteins, called hMSH2.
Expression of hMSH2 in E. coli causes a dominant mutator
phenotype, suggesting that hMSH2, like other
divergent ...
The centromere, on which kinetochore proteins assemble, ensures precise chromosome segregation. Centromeres are largely specified by the histone H3 variant CENP-A (also known as Cse4 in yeasts). Structurally, centromere ...
Jain, Kavita; Stephan, Wolfgang(Genetics Society of America, 2015)
We consider an infinitely large population under stabilizing selection and mutation in which the allelic effects determining a polygenic trait vary between loci. We obtain analytical expressions for the stationary genetic ...
