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Characterization of poly(ADP-ribosyl)ated domains of rat pachytene chromatin

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dc.contributor.author Satyanarayana, Karuturi
dc.contributor.author Rao, M R S
dc.date.accessioned 2012-02-23T11:11:53Z
dc.date.available 2012-02-23T11:11:53Z
dc.date.issued 1989-08-01
dc.identifier 0264-6021 en_US
dc.identifier.citation Biochemical Journal 261(3), 775-786 (1989) en_US
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/499
dc.description Restricted Access en_US
dc.description.abstract Poly(ADP-ribosyl)ation of nuclear proteins was several-fold higher in the pachytene spermatocytes than in the premeiotic germ cells of the rat. Among the histones of the pachytene nucleus, histone subtypes H2A, H1 and H3 were poly(ADP-ribosyl)ated. Based on the immunoaffinity fractionation procedure of Malik, Miwa, Sugimara & Smulson [(1983) Proc. Natl. Acad. Sci. U.S.A. 80, 2554-2558] we have fractionated DNAase-II-solubilized chromatin into poly(ADP-ribosyl)ated chromatin (PAC) and non-poly(ADP-ribosyl)ated chromatin (non-PAC) domains on an anti-[poly(ADP-ribose)] IgG affinity matrix. Approx. 2.5% of the pachytene chromatin represented the PAC domains. A significant amount of [alpha-32P]dATP-labelled pachytene chromatin (labelled in vitro) was bound to the affinity matrix. The DNA of pachytene PAC domains had internal strand breaks, significant length of gaps and ligatable ends, namely 5'-phosphoryl and 3'-hydroxyl termini. On the other hand, the PAC domains from 18 h regenerating liver had very few gaps, if any. The presence of gaps in the pachytene PAC DNA was also evident from thermal denaturation studies. Although many of the polypeptides were common to the PAC domains of both pachytene and regenerating liver, the DNA sequences associated with these domains were quite different. A 20 kDa protein and the testis-specific histone H1t were selectively enriched in the pachytene PAC domains. The pachytene PAC domains also contained approx. 10% of the messenger coding sequences present in the DNAase-II-solubilized chromatin. The pachytene PAC domains, therefore, may represent highly enriched DNA-repair domains of the pachytene nucleus. en_US
dc.description.uri http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1138899/?tool=pubmed en_US
dc.language.iso en en_US
dc.publisher Portland Press en_US
dc.rights © 1989 The Biochemical Society en_US
dc.subject Animals en_US
dc.subject Base Sequence en_US
dc.subject Chromatin/isolation & purification en_US
dc.subject DNA Repair en_US
dc.subject Liver-analysis en_US
dc.subject Male en_US
dc.subject Meiosis en_US
dc.subject Nucleoside Diphosphate Sugars/isolation & purification en_US
dc.subject Poly Adenosine Diphosphate Ribose/isolation & purification en_US
dc.subject Rats en_US
dc.subject Spermatocytes-analysis en_US
dc.subject Synaptonemal Complex en_US
dc.title Characterization of poly(ADP-ribosyl)ated domains of rat pachytene chromatin en_US
dc.type Article en_US


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