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Isolation and Characterization of Binding Proteins for Retinol from the Cytosol, Nucleosol and Chromatin of the Oviduct Magnum of Laying Hens

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dc.contributor.author Rao, M R S
dc.contributor.author Prasad, V R
dc.contributor.author Padmanaban, Govindarajan
dc.contributor.author Ganguly, Jagannath
dc.date.accessioned 2012-02-28T06:59:09Z
dc.date.available 2012-02-28T06:59:09Z
dc.date.issued 1979
dc.identifier 0264-6021 en_US
dc.identifier.citation Biochemical Journal 183(3), 501–506 (1979) en_US
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/509
dc.description Restricted Access en_US
dc.description.abstract Protein fractions that bind retinol were isolated from the cytosol, nucleosol and chromatin of the oviduct magnum of laying hens. The proteins isolated from the three sources showed similar elution profiles on chromatography through Sephadex G-75 and G-50 columns, and comparable mobility during electrophoresis on sodium dodecyl sulphate/polyacrylamide gels. Their molecular weights were calculated to be around 14500. When oviducts from vitamin A-depleted and vitamin A-repleted immature chicks given oestrogen injections for 6 consecutive days were incubated with [3H]retinyl acetate, uptake of the radioactivity in the nuclei of the vitamin A-depleted tissue was severalfold higher than that in the nuclei from the vitamin A-repleted tissue. en_US
dc.description.uri www.ncbi.nlm.nih.gov/pmc/articles/PMC1161629/ en_US
dc.language.iso en en_US
dc.publisher The Biochemical Society en_US
dc.rights © 1979 The Biochemical Society en_US
dc.subject Animals en_US
dc.subject Cell Nucleus - analysis en_US
dc.subject Chickens en_US
dc.subject Chromatin - analysis en_US
dc.subject Chromatography en_US
dc.subject Gel en_US
dc.subject Cytosol - analysis en_US
dc.subject Estrogens - pharmacology en_US
dc.subject Oviducts - analysis en_US
dc.subject drug effects en_US
dc.subject Retinol-Binding Proteins - isolation & purification en_US
dc.subject Vitamin A - metabolism en_US
dc.subject Vitamin A Deficiency - metabolism en_US
dc.title Isolation and Characterization of Binding Proteins for Retinol from the Cytosol, Nucleosol and Chromatin of the Oviduct Magnum of Laying Hens en_US
dc.type Article en_US


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