Faculty publications (MBGU): Recent submissions

Now showing items 61-80 of 189

Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On

Shetty, Deeti K.; Shetty, Ronak; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Human embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On ...
Generation of a transgenic human embryonic stem cell line ectopically expressing the endosomal protein Asrij that regulates pluripotency in mouse embryonic stem cells: BJNhem20-Asrij

Shetty, Deeti K.; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Asrij is an endocytic protein expressed in mouse embryonic stem cells (mESCs) and is essential for maintenance of stemness of mESCs (Mukhopadhyay et al., 2003; Sinha et al., 2013). Its human ortholog named Ovarian Carcinoma ...
Generation of human embryonic stem cell line expressing green fluorescent protein

Shetty, Ronak; Shetty, Deeti; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Human embryonic stem cell line BJNhem20 pCAG-EGFP was generated using non-viral method (Fig. 1). The construct pCAG-EGFP was transfected using microporation procedure (C) 2015 The Authors. Published by Elsevier B.V.
Controlled Inhibition of the Mesenchymal Stromal Cell Pro-inflammatory Secretome via Microparticle Engineering

Ranganath, Sudhir H.; Tong, Zhixiang; Levy, Oren; Martyn, Keir; Karp, Jeffrey M.; Inamdar, Maneesha S. (Cell Press, 2016)

Mesenchymal stromal cells (MSCs) are promising therapeutic candidates given their potent immunomodulatory and anti-inflammatory secretome. However, controlling the MSC secretome post-transplantation is considered a major ...
Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-20

Shetty, Deeti K.; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Ovarian carcinoma immuno-reactive antigen domain containing 1(OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-20. The line was generated ...
Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato

Shetty, Deeti K.; Inamdar, Maneesha S. (Elsevier Science Bv, 2016)

Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity ...
Mode of Action of a Designed Antimicrobial Peptide: High Potency against Cryptococcus neoformans

Datta, Aritreyee; Yadav, Vikas; Ghosh, Anirban; Choi, Jaesun; Bhattacharyya, Dipita; Kar, Rajiv K.; Ilyas, Humaira; Dutta, Arkajyoti; An, Eunseol; Mukhopadhyay, Jayanta; Lee, Dongkuk; Sanyal, Kaustuv; Ramamoorthy, Ayyalusamy; Bhunia, Anirban (Cell Press, 2016)

There is a significant need for developing compounds that kill Cryptococcus neoformans, the fungal pathogen that causes meningoencephalitis in immunocompromised individuals. Here, we report the mode of action of a designed ...
Repeat-Associated Fission Yeast-Like Regional Centromeres in the Ascomycetous Budding Yeast Candida tropicalis

Chatterjee, Gautam; Sankaranarayanan, Sundar Ram; Guin, Krishnendu; Thattikota, Yogitha; Padmanabhan, Sreedevi; Siddharthan, Rahul; Sanyal, Kaustuv (Public Library of Science, 2016)

The centromere, on which kinetochore proteins assemble, ensures precise chromosome segregation. Centromeres are largely specified by the histone H3 variant CENP-A (also known as Cse4 in yeasts). Structurally, centromere ...
Product Release Pathways in Human and Plasmodium falciparum Phosphoribosyltransferase

Karmakar, Tarak; Roy, Sourav; Balararn, Hemalatha; Prakash, Meher K.; Balasubramanian, Sundaram (American Chemical Society, 2016)

Atomistic molecular dynamics (MD) simulations coupled with the metadynamics technique were carried out to delineate the product (PPi.2Mg and IMP) release mechanisms from the active site of both human (Hs) and Plasmodium ...
Connecting Active-Site Loop Conformations and Catalysis in Triosephosphate Isomerase: Insights from a Rare Variation at Residue96 in the Plasmodial Enzyme

Pareek, Vidhi; Samanta, Moumita; Joshi, Niranjan V.; Balaram, Hemalatha; Murthy, Mathur R. N.; Balaram, Padmanabhan (Wiley-V C H Verlag Gmbh, 2016)

Despite extensive research into triosephosphate isomerases (TIMs), there exists a gap in understanding of the remarkable conjunction between catalytic loop-6 (residues 166-176) movement and the conformational flip of Glu165 ...
Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase

Kumar, Sanjeev; Prakash, Sunita; Gupta, Kallol; Dongre, Aparna; Balaram, Padmanabhan; Balaram, Hemalatha (Nature Publishing Group, 2016)

Protein ageing is often mediated by the formation of succinimide intermediates. These short-lived intermediates derive from asparaginyl deamidation and aspartyl dehydration and are rapidly converted into beta-aspartyl or ...
Exquisite Modulation of the Active Site of Methanocaldococcus jannaschii Adenylosuccinate Synthetase in Forward Reaction Complexes

Karnawat, Vishakha; Mehrotra, Sonali; Balaram, Hemalatha; Puranik, Mrinalini (American Chemical Society, 2016)

In enzymes that conduct complex reactions involving several substrates and chemical transformations, the active Site must reorganize at each step to complement the transition state of that chemical step. Adenylosuccinate ...
Structural and dynamical correlations in PfHGXPRT oligomers: A molecular dynamics simulation study

Karmakar, Tarak; Roy, Sourav; Balaram, Hemalatha; Balasubramanian, Sundaram (Taylor & Francis Inc, 2016)

PfHGXPRT is a key enzyme involved in purine nucleotide salvage pathway of the malarial parasite, Plasmodium falciparum. Atomistic molecular dynamics simulations have been performed on two types of PfHGXPRT dimers (D1 and ...
Functional Analysis of a Novel Connexin30 Mutation in a Large Family with Hearing Loss, Pesplanus, Ichthyosis, Cutaneous Nodules, and Keratoderma

Pandey, Nishtha; Xavier, Dennis F.; Chatterjee, Arunima; Mani, Ram-Shankar; Hiremagalore, Ravi; Tharakan, Ajith; Rajashekhar, B.; Anand, Anuranjan (Wiley-Blackwell, 2016)

Mutations in the gap-junction gene Cx30 (Connexin30, GJB6) are a known cause of hearing loss. Here, we report our findings on a large multigeneration family in which severe to profound sensorineural hearing impairment is ...
Genetic diversity and proviral DNA load in different neural compartments of HIV-1 subtype C infection

Mishra, Mamata; Varghese, Rebu K.; Verma, Anjali; Das, Sutanuka; Aguiar, Renato Santana; Tanuri, Amilcar; Mahadevan, Anita; Shankar, Susarla K.; Satishchandra, Parthasarathy; Ranga, Udaykumar (Springer, 2015)

In India, the low prevalence of HIV-associated dementia (HAD) in the Human immunodeficiency virus type 1 (HIV-1) subtype C infection is quite paradoxical given the high-rate of macrophage infiltration into the brain. Whether ...
Stealth anti-CD4 conjugated immunoliposomes with dual antiretroviral drugs - Modern Trojan horses to combat HIV

Ramana, Lakshmi Narashimhan; Sharma, Shilpee; Sethuraman, Swaminathan; Ranga, Udaykumar; Krishnan, Uma Maheswari (Elsevier Science Bv, 2015)

Highly active antiretroviral therapy (HAART) is the currently employed therapeutic intervention against AIDS where a drug combination is used to reduce the viral load. The present work envisages the development of a stealth ...
Multispectral Bayesian reconstruction technique for real-time two color fluorescence microscopy

Dilipkumar, Shilpa; Manjithaya, Ravi; Mondal, Partha Pratim (Royal Society of Chemistry, 2015)

We have developed a real-time imaging method for two-color wide-field fluorescence microscopy using a combined approach that integrates multi-spectral imaging and Bayesian image reconstruction technique. To enable simultaneous ...
Rationally Designed Peptidomimetic Modulators of A beta Toxicity in Alzheimer's Disease

Rajasekhar, K.; Suresh, S. N.; Manjithaya, Ravi; Govindaraju, T. (Nature Publishing Group, 2015)

Alzheimer's disease is one of the devastating illnesses mankind is facing in the 21st century. The main pathogenic event in Alzheimer's disease is believed to be the aggregation of the beta-amyloid (A beta) peptides into ...
Fluorescence microscopy: A tool to study autophagy

Rai, Shashank; Manjithaya, Ravi (American Institute of Physics, 2015)

Autophagy is a cellular recycling process through which a cell degrades old and damaged cellular components such as organelles and proteins and the degradation products are reused to provide energy and building blocks. ...
Broad Spectrum Antibacterial and Antifungal Polymeric Paint Materials: Synthesis, Structure-Activity Relationship, and Membrane-Active Mode of Action

Hoque, Jiaul; Akkapeddi, Padma; Yadav, Vikas; Manjunath, Goutham B.; Uppu, Divakara S. S. M.; Konai, Mohini M.; Yarlagadda, Venkateswarlu; Sanyal, Kaustuv; Haldar, Jayanta (American Chemical Society, 2015)

Microbial attachment and subsequent colonization onto surfaces lead to the spread of deadly community-acquired and hospital-acquired (nosocomial) infections. Noncovalent immobilization of water insoluble and organo-soluble ...

Now showing items 61-80 of 189

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