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Promoter-proximal transcription factor binding is transcriptionally active when coupled with nucleosome repositioning in immediate vicinity

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dc.contributor.author Yadav, Vinod Kumar
dc.contributor.author Thakur, Ram Krishna
dc.contributor.author Eckloff, Bruce
dc.contributor.author Baral, Aradhita
dc.contributor.author Singh, Ankita
dc.contributor.author Halder, Rashi
dc.contributor.author Kumar, Akinchan
dc.contributor.author Alam, Mohammad Parwez
dc.contributor.author Kundu, Tapas Kumar
dc.contributor.author Pandita, Raj
dc.contributor.author Pandita, Tej K.
dc.contributor.author Wieben, Eric D.
dc.contributor.author Chowdhury, Shantanu
dc.date.accessioned 2017-02-17T05:09:15Z
dc.date.available 2017-02-17T05:09:15Z
dc.date.issued 2014
dc.identifier.citation Yadav, VK; Thakur, RK; Eckloff, B; Baral, A; Singh, A; Halder, R; Kumar, A; Alam, MP; Kundu, TK; Pandita, R; Pandita, TK; Wieben, ED; Chowdhury, S, Promoter-proximal transcription factor binding is transcriptionally active when coupled with nucleosome repositioning in immediate vicinity. Nucleic Acids Research 2014, 42 (15) 9602-9611, http://dx.doi.org/10.1093/nar/gku596 en_US
dc.identifier.citation Nucleic Acids Research en_US
dc.identifier.citation 42 en_US
dc.identifier.citation 15 en_US
dc.identifier.issn 0305-1048
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/2327
dc.description Open Access en_US
dc.description.abstract Previous studies have analyzed patterns of transcription, transcription factor (TF) binding or mapped nucleosome occupancy across the genome. These suggest that the three aspects are genetically connected but the cause and effect relationships are still unknown. For example, physiologic TF binding studies involve many TFs, consequently, it is difficult to assign nucleosome reorganization to the binding site occupancy of any particular TF. Therefore, several aspects remain unclear: does TF binding influence nucleosome (re) organizations locally or impact the chromatin landscape at a more global level; are all or only a fraction of TF binding a result of reorganization in nucleosome occupancy and do all TF binding and associated changes in nucleosome occupancy result in altered gene expression? With these in mind, following characterization of two states (before and after induction of a single TF of choice) we determined: (i) genomic binding sites of the TF, (ii) promoter nucleosome occupancy and (iii) transcriptome profiles. Results demonstrated that promoter-proximal TF binding influenced expression of the target gene when it was coupled to nucleosome repositioning at or close to its binding site in most cases. In contrast, only in few cases change in target gene expression was found when TF binding occurred without local nucleosome reorganization. en_US
dc.description.uri 1362-4962 en_US
dc.description.uri http://dx.doi.org/10.1093/nar/gku596 en_US
dc.language.iso English en_US
dc.publisher Oxford University Press en_US
dc.rights @Oxford University Press, 2014 en_US
dc.subject Biochemistry & Molecular Biology en_US
dc.subject Nm23/Nucleoside Diphosphate Kinase en_US
dc.subject Saccharomyces-Cerevisiae en_US
dc.subject Histone Modifications en_US
dc.subject Cancer Metastasis en_US
dc.subject Gene-Expression en_US
dc.subject Cell Invasion en_US
dc.subject Human Genome en_US
dc.subject DNA-Binding en_US
dc.subject Chromatin en_US
dc.subject Mechanisms en_US
dc.title Promoter-proximal transcription factor binding is transcriptionally active when coupled with nucleosome repositioning in immediate vicinity en_US
dc.type Article en_US


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