dc.contributor.author |
Kundu, Partha P.
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|
dc.contributor.author |
Bhowmick, Tuhin
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|
dc.contributor.author |
Swapna, Ganduri
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|
dc.contributor.author |
Kumar, G. V. Pavan
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|
dc.contributor.author |
Nagaraja, V.
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|
dc.contributor.author |
Narayana, Chandrabhas
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dc.date.accessioned |
2017-02-21T09:53:24Z |
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dc.date.available |
2017-02-21T09:53:24Z |
|
dc.date.issued |
2014 |
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dc.identifier.citation |
Kundu, PP; Bhowmick, T; Swapna, G; Kumar, GVP; Nagaraja, V; Narayana, C, Allosteric Transition Induced by Mg2+ Ion in a Transactivator Monitored by SERS. Journal of Physical Chemistry B 2014, 118 (20) 5322-5330, http://dx.doi.org/10.1021/jp5000733 |
en_US |
dc.identifier.citation |
Journal of Physical Chemistry B |
en_US |
dc.identifier.citation |
118 |
en_US |
dc.identifier.citation |
20 |
en_US |
dc.identifier.issn |
1520-6106 |
|
dc.identifier.uri |
https://libjncir.jncasr.ac.in/xmlui/10572/2596 |
|
dc.description |
Restricted Access |
en_US |
dc.description.abstract |
We demonstrate the utility of the surface-enhanced Raman spectroscopy (SERS) to monitor conformational transitions in protein upon ligand binding. The changes in protein's secondary and tertiary structures were monitored using amide and aliphatic/aromatic side chain vibrations. Changes in these bands are suggestive of the stabilization of the secondary and tertiary structure of transcription activator protein C in the presence of Mg2+ ion, whereas the spectral fingerprint remained unaltered in the case of a mutant protein, defective in Mg2+ binding. The importance of the acidic residues in Mg2+ binding, which triggers an overall allosteric transition in the protein, is visualized in the molecular model. The present study thus opens up avenues toward the application of SERS as a potential tool for gaining structural insights into the changes occurring during conformational transitions in proteins. |
en_US |
dc.description.uri |
http://dx.doi.org/10.1021/jp5000733 |
en_US |
dc.language.iso |
English |
en_US |
dc.publisher |
American Chemical Society |
en_US |
dc.rights |
@American Chemical Society, 2014 |
en_US |
dc.subject |
Physical Chemistry |
en_US |
dc.subject |
Surface-Enhanced Raman |
en_US |
dc.subject |
Label-Free Detection |
en_US |
dc.subject |
Mu C-Protein |
en_US |
dc.subject |
Conformational-Changes |
en_US |
dc.subject |
Bacteriophage-Mu |
en_US |
dc.subject |
Single-Molecule |
en_US |
dc.subject |
Amino-Acids |
en_US |
dc.subject |
Secondary Structure |
en_US |
dc.subject |
Silver Electrode |
en_US |
dc.subject |
Colloidal Silver |
en_US |
dc.title |
Allosteric Transition Induced by Mg2+ Ion in a Transactivator Monitored by SERS |
en_US |
dc.type |
Article |
en_US |