DSpace Repository

Allosteric Transition Induced by Mg2+ Ion in a Transactivator Monitored by SERS

Show simple item record

dc.contributor.author Kundu, Partha P.
dc.contributor.author Bhowmick, Tuhin
dc.contributor.author Swapna, Ganduri
dc.contributor.author Kumar, G. V. Pavan
dc.contributor.author Nagaraja, V.
dc.contributor.author Narayana, Chandrabhas
dc.date.accessioned 2017-02-21T09:53:24Z
dc.date.available 2017-02-21T09:53:24Z
dc.date.issued 2014
dc.identifier.citation Kundu, PP; Bhowmick, T; Swapna, G; Kumar, GVP; Nagaraja, V; Narayana, C, Allosteric Transition Induced by Mg2+ Ion in a Transactivator Monitored by SERS. Journal of Physical Chemistry B 2014, 118 (20) 5322-5330, http://dx.doi.org/10.1021/jp5000733 en_US
dc.identifier.citation Journal of Physical Chemistry B en_US
dc.identifier.citation 118 en_US
dc.identifier.citation 20 en_US
dc.identifier.issn 1520-6106
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/2596
dc.description Restricted Access en_US
dc.description.abstract We demonstrate the utility of the surface-enhanced Raman spectroscopy (SERS) to monitor conformational transitions in protein upon ligand binding. The changes in protein's secondary and tertiary structures were monitored using amide and aliphatic/aromatic side chain vibrations. Changes in these bands are suggestive of the stabilization of the secondary and tertiary structure of transcription activator protein C in the presence of Mg2+ ion, whereas the spectral fingerprint remained unaltered in the case of a mutant protein, defective in Mg2+ binding. The importance of the acidic residues in Mg2+ binding, which triggers an overall allosteric transition in the protein, is visualized in the molecular model. The present study thus opens up avenues toward the application of SERS as a potential tool for gaining structural insights into the changes occurring during conformational transitions in proteins. en_US
dc.description.uri http://dx.doi.org/10.1021/jp5000733 en_US
dc.language.iso English en_US
dc.publisher American Chemical Society en_US
dc.rights @American Chemical Society, 2014 en_US
dc.subject Physical Chemistry en_US
dc.subject Surface-Enhanced Raman en_US
dc.subject Label-Free Detection en_US
dc.subject Mu C-Protein en_US
dc.subject Conformational-Changes en_US
dc.subject Bacteriophage-Mu en_US
dc.subject Single-Molecule en_US
dc.subject Amino-Acids en_US
dc.subject Secondary Structure en_US
dc.subject Silver Electrode en_US
dc.subject Colloidal Silver en_US
dc.title Allosteric Transition Induced by Mg2+ Ion in a Transactivator Monitored by SERS en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account