DSpace Repository

Sanguinarine Interacts with Chromatin, Modulates Epigenetic Modifications, and Transcription in the Context of Chromatin

Show simple item record

dc.contributor.author B, Ruthrotha Selvi
dc.contributor.author Pradhan, Suman Kalyan
dc.contributor.author Shandilya, Jayasha
dc.contributor.author Das, Chandrima
dc.contributor.author Sailaja, Badi Sri
dc.contributor.author G, Naga Shankar
dc.contributor.author Gadad, Shrikanth S
dc.contributor.author Reddy, Ashok
dc.contributor.author Dasgupta, Dipak
dc.contributor.author Kundu, Tapas K
dc.date.accessioned 2012-02-13T10:30:26Z
dc.date.available 2012-02-13T10:30:26Z
dc.date.issued 2009-02-27
dc.identifier 1074-5521 en_US
dc.identifier.citation Chemistry & Biology 16(2), 203-216 (2009) en_US
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/394
dc.description Restricted Access en_US
dc.description.abstract DNA-binding anticancer agents cause alteration in chromatin structure and dynamics. We report the dynamic interaction of the DNA intercalator and potential anticancer plant alkaloid, sanguinarine (SGR), with chromatin. Association of SGR with different levels of chromatin structure was enthalpy driven with micromolar dissociation constant. Apart from DNA, it binds with comparable affinity with core histones and induces chromatin aggregation. The dual binding property of SGR leads to inhibition of core histone modifications. Although it potently inhibits H3K9 methylation by G9a in vitro, H3K4 and H3R17 methylation are more profoundly inhibited in cells. SGR inhibits histone acetylation both in vitro and in vivo. It does not affect the in vitro transcription from DNA template but significantly represses acetylation-dependent chromatin transcription. SGR-mediated repression of epigenetic marks and the alteration of chromatin geography (nucleography) also result in the modulation of global gene expression. These data, conclusively, show an anticancer DNA binding intercalator as a modulator of chromatin modifications and transcription in the chromatin context. en_US
dc.description.sponsorship Department of Atomic Energy, Government of India, Jawaharlal Nehru Centre for Advanced Scientific Research en_US
dc.description.uri http://dx.doi.org/10.1016/j.chembiol.2008.12.006 en_US
dc.language.iso en en_US
dc.publisher Cell Press en_US
dc.rights © 2009 Elsevier Ltd en_US
dc.subject Global Gene-Expression en_US
dc.subject Benzophenanthridine Alkaloids en_US
dc.subject Cell-Cycle en_US
dc.subject Protein en_US
dc.subject Inhibitor en_US
dc.subject Apoptosis en_US
dc.subject Acetyltransferase en_US
dc.subject Activation en_US
dc.subject Represses en_US
dc.subject Phosphorylation en_US
dc.title Sanguinarine Interacts with Chromatin, Modulates Epigenetic Modifications, and Transcription in the Context of Chromatin en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account