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Surface-Enhanced Raman Scattering Studies of Human Transcriptional Coactivator p300

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dc.contributor.author Kumar, G V Pavan
dc.contributor.author Reddy, B A Ashok
dc.contributor.author Arif, Mohammed
dc.contributor.author Kundu, Tapas K
dc.contributor.author Narayana, Chandrabhas
dc.date.accessioned 2012-03-06T05:46:24Z
dc.date.available 2012-03-06T05:46:24Z
dc.date.issued 2006-08-24
dc.identifier 1520-6106 en_US
dc.identifier.citation Journal of Physical Chemistry B 110(33), 16787-16792 (2006) en_US
dc.identifier.uri https://libjncir.jncasr.ac.in/xmlui/10572/562
dc.description Restricted Access en_US
dc.description.abstract We report for the first time the surface-enhanced Raman scattering (SERS) studies on p300, a large multidomain transcriptional coactivator protein. Vibration spectral analysis has been performed in an attempt to understand the structure of the p300 in the absence of its crystal structure. Strong Raman bands associated with amides I-III have been observed in the protein spectra. This has been confirmed by performing SERS on deuterated p300. We also observe Raman bands associated with the alpha-helix, tryptophan, phenylalanine, tyrosine, and histidine. These bands will provide an ideal tool to study the drug-protein interactions in therapeutics using SERS. We have successfully demonstrated the chloride ion effect on the SERS of p300. The Raman intensity increases in the SERS spectra upon addition of chloride ion along with appearance of new modes. We have developed a new method, namely, the "sandwich technique", which could be used to perform SERS experiments on proteins in dry conditions. en_US
dc.description.uri http://dx.doi.org/10.1021/jp063071e en_US
dc.language.iso en en_US
dc.publisher American Chemical Society en_US
dc.rights © 2006 American Chemical Society en_US
dc.subject Double-Stranded Dna en_US
dc.subject Histone Acetyltransferase en_US
dc.subject Colloidal Silver en_US
dc.subject Amino-Acids en_US
dc.subject Spectroscopy en_US
dc.subject Proteins en_US
dc.subject Cbp en_US
dc.subject Domain en_US
dc.subject Acetylation en_US
dc.subject Adsorption en_US
dc.title Surface-Enhanced Raman Scattering Studies of Human Transcriptional Coactivator p300 en_US
dc.type Article en_US


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