Abstract:
"The current study focuses on the preliminary characterisation of a purine nucleotide cycle
enzyme, AMP deaminase (AMPD hereafter) from Plasmodium falciparum. Purine nucleotide
cycle performs inter-conversion of IMP and AMP with the release of fumarate and ammonia
as by-products, which also have physiological consequences. The pathway plays a chief role
in maintaining adenylate energy charge ratio, which is critical for cell survival. Cloning,
expression and preliminary characterization of the enzyme has been reported in this thesis."
